Recombinant Mouse Anti-AFP Antibody (VS3-FY68)
CAT#: VS3-FY68
This product is a recombinant mouse antibody that recognizes human AFP. AFP mouse Monoclonal antibody detects endogenous AFP protein protein. It can detect a band of approximately 70 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- AFP Mouse Monoclonal antibody detects endogenous AFP protein.
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- 70 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/2000
Immunohistochemistry:1/50-300
Immunofluorescence:1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| TAB-713 | Humanized Anti-AFP Recombinant Antibody (TAB-713) | IF, IP, Neut, FuncS, ELISA, FC, WB | Humanized (from mouse) IgG1, κ |
| CAT | Product Name | Application | Type |
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| AGTO-G071G | Anti-AFP immunotoxin 80G (F (ab')2)-Gel | Cytotoxicity assay, Function study | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-0099 | Hi-Affi™ Rabbit Anti-AFP Recombinant Antibody (clone DS99AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0017-YC-S(P) | Mouse Anti-AFP Recombinant Antibody (clone AF5); scFv Fragment | WB | Mouse scFv |
| HPAB-0296-FY-S(P) | Mouse Anti-AFP Recombinant Antibody scFv Fragment (HPAB-0296-FY-S(P)) | ELISA, IF, IHC | Mouse scFv |
| HPAB-0297-FY-S(P) | Mouse Anti-AFP Recombinant Antibody scFv Fragment (HPAB-0297-FY-S(P)) | ELISA, IF, IHC | Mouse scFv |
| HPAB-0298-FY-S(P) | Mouse Anti-AFP Recombinant Antibody scFv Fragment (HPAB-0298-FY-S(P)) | ELISA, IF, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0017-YC-F(E) | Mouse Anti-AFP Recombinant Antibody (clone AF5); Fab Fragment | WB | Mouse Fab |
| HPAB-0296-FY-F(E) | Mouse Anti-AFP Recombinant Antibody Fab Fragment (HPAB-0296-FY-F(E)) | ELISA, IF, IHC | Mouse Fab |
| HPAB-0297-FY-F(E) | Mouse Anti-AFP Recombinant Antibody Fab Fragment (HPAB-0297-FY-F(E)) | ELISA, IF, IHC | Mouse Fab |
| HPAB-0298-FY-F(E) | Mouse Anti-AFP Recombinant Antibody Fab Fragment (HPAB-0298-FY-F(E)) | ELISA, IF, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1628-CN | Rabbit Anti-AFP Polyclonal Antibody (MRO-1628-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1629-CN | Rabbit Anti-AFP Polyclonal Antibody (MRO-1629-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ309 | Mouse Anti-AFP Recombinant Antibody (clone PT0679) | WB | |
| VS3-CJ310 | Mouse Anti-AFP Recombinant Antibody (VS3-CJ310) | WB | |
| VS-0923-FY10 | Recombinant Mouse Anti-AFP Antibody (VS-0923-FY10) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-713 | Afuco™ Anti-AFP ADCC Recombinant Antibody, ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY30 | Mouse Anti-AFP Recombinant Antibody (clone 143CT50.11.8) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC295 | AbPlus™ Anti-AFP Magnetic Beads (VS-0724-YC295) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC15 | Mouse Anti-AFP Recombinant Antibody (VS-0924-YC15) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG2a |
| VS-0924-YC17 | Rabbit Anti-AFP Antibody (VS-0924-YC17) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY10 | Mouse Anti-NHP AFP Recombinant Antibody (clone SPM334) | IHC, ICC, FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY55 | Anti-AFP Immunohistochemistry Kit | IHC | |
| VS-0525-XY206 | Anti-Human AFP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY64 | Mouse Anti-AFP scFv-Fc Chimera (VS-0425-FY64) | WB, FC, IF | Mouse IgG1, scFv-Fc |
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