Recombinant Mouse Anti-AFP Detection Antibody (clone 10F12)
CAT#: VS3-WK1669
This antibody is a mouse recombinant antibody against human AFP. The sandwich ELISA was established using matched antibody pair 3C11 and 10F12. The clone 10F12 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human AFP protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2a.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
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Specifications
- Immunogen
- Recombinant human AFP protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human AFP
- Species Reactivity
- Human
- Clone
- 10F12
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA
- Gene ID
- 174
- UniProt ID
- P02771
- Long Name
- Alpha Fetoprotein
- Sequence Similarities
- Belongs to the ALB/AFP/VDB family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Protein Refseq
- NP_001125.1
- Function
- Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| HPAB-0296-FY-F(E) | Mouse Anti-AFP Recombinant Antibody Fab Fragment (HPAB-0296-FY-F(E)) | ELISA, IF, IHC | Mouse Fab |
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| CAT | Product Name | Application | Type |
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