Recombinant Mouse Anti-AFP Detection Antibody (clone 10F12) (CAT#: VS3-WK1669)
This antibody is a mouse recombinant antibody against human AFP. The sandwich ELISA was established using matched antibody pair 3C11 and 10F12. The clone 10F12 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human AFP protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2a.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
(Immunohistochemical staining of human placenta shows strong positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
Specifications
- Immunogen
- Recombinant human AFP protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human AFP
- Species Reactivity
- Human
- Clone
- 10F12
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA
- Gene ID
- 174
- UniProt ID
- P02771
- Long Name
- Alpha Fetoprotein
- Sequence Similarities
- Belongs to the ALB/AFP/VDB family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
- Protein Refseq
- NP_001125.1
- Function
- Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-011 | Recombinant Anti-Human AFP VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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