Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3)

Name: Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3)
SKU: VS3-FY466
Rating: 5 (1 reviews)

CAT#: VS3-FY466

This product is a recombinant rabbit antibody that recognizes human, mouse and rat E2F1 protein. It can detect a band of approximately 47 kDa (observedd molecular weight: 70 kDa).

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg

Normal Tissue Figure 3 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg

Normal Tissue Figure 4 IHC staining of human colon

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg

Normal Tissue Figure 7 Liver

Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg

Normal Tissue Figure 9 Testis

Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg

Normal Tissue Figure 10 Bone marrow

Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1

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Figure 1. Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) in WB.

Western blot analysis of E2F1 in C6, 3T3 lysates using E2F1 antibody.

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Specifications

Immunogen
A synthetic peptide of human E2F1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat E2F1

Species Reactivity
Human, Mouse, Rat

Clone
R03-5G3

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 47 kDa; Observed MW: 70 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

Gene ID
1869

UniProt ID
Q01094

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Related Diseases

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY466.pdf

MSDS

MSDS - VS3-FY466.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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