Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) (CAT#: VS3-FY466)
This product is a recombinant rabbit antibody that recognizes human, mouse and rat E2F1 protein. It can detect a band of approximately 47 kDa (observedd molecular weight: 70 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) in WB.
Western blot analysis of E2F1 in C6, 3T3 lysates using E2F1 antibody.
Specifications
- Immunogen
- A synthetic peptide of human E2F1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat E2F1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5G3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 47 kDa; Observed MW: 70 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
- Gene ID
- 1869
- UniProt ID
- Q01094
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1080 | Hi-Affi™ Rabbit Anti-E2F1 Recombinant Antibody (clone DS1080AB) | WB, ICC, IF, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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