Recombinant Rabbit Anti-GAD2 Detection Antibody (clone R86-6O-6) (CAT#: VS3-WK1835)
This antibody is a rabbit recombinant antibody against human GAD2. It was developed by immunization of rabbit with recombinant human GAD2 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R46-7A-6 and R86-6O-6 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R86-6O-6 can be used in ELISA analysis to identify human GAD2.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78177/163309_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78177/163309_B_9_5.jpg
Figure 2 Pancreas
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78177/163309_A_3_3.jpg
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78177/163309_A_3_3.jpg
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136750-GAD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136750-GAD2
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136750-GAD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136750-GAD2
Specifications
- Immunogen
- Recombinant human GAD2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GAD2
- Species Reactivity
- Human
- Clone
- R86-6O-6
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Glutamate Decarboxylase 2; Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa); Glutamate Decarboxylase 65 KDa Isoform; 5 KDa Glutamic Acid Decarboxylase; EC 4.1.1.15; GAD-65
- Gene ID
- 2572
- UniProt ID
- Q05329
- Long Name
- Glutamate Decarboxylase 2
- Sequence Similarities
- Belongs to the group II decarboxylase family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse
- Post Translation Modifications
- Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
- Protein Refseq
- NP_000809.1
- Function
- Catalyzes the production of GABA.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1147z | Mouse Anti-GAD2 Recombinant Antibody (clone 28G2) | WB, ELISA, FC | Mouse IgG1 |
| MOB-0421ZL | Rabbit anti-GAD2 polyclonal Antibody | WB | |
| MOB-2430MZ | Recombinant Mouse Anti-Rat GAD2 Antibody (clone HBE-7) | IHC-FoFr, IHC-Fr, IHC-P, WB | Mouse antibody |
| VS1-YJ63 | Mouse Anti-GAD2 Recombinant Antibody (clone 144) | WB, FC, IP | Mouse IgG1 |
| VS3-WK1834 | Recombinant Rabbit Anti-GAD2 Capture Antibody (clone R46-7A-6) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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