Recombinant Rabbit Anti-Hnf4A Antibody (clone R02-7J6) (CAT#: VS3-FY692)
This product is a recombinant rabbit antibody that recognizes Hnf4A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R02-7J6 is specific for mouse Hnf4A. The antigen is mouse hnf-4-alpha recombinant protein.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
Figure 2 Duodenum
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
Figure 3 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
Figure 4 Colon
(Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
(Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
Figure 6 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
Specifications
- Immunogen
- Mouse HNF-4-alpha recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse Hnf4A
- Species Reactivity
- Mouse
- Clone
- R02-7J6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 53 kDa; Observed MW: 53 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Hepatocyte Nuclear Factor 4 Alpha; Nuclear Receptor Subfamily 2 Group A Member 1; Transcription Factor HNF-4; Transcription Factor 14; TCF-14; TCF14; NR2A1; HNF4; Hepatocyte Nuclear Factor 4-Alpha; Hepatic Nuclear Factor 4 Alpha; HNF4alpha10/11/12
- Gene ID
- 3172
- UniProt ID
- P41235
- Cellular Localization
- Nucleus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1642 | Rabbit Anti-HNF4A Recombinant Antibody (clone DS1642AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
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