Recombinant Mouse Anti-ICAM1 Antibody (clone 1G3-9D5-6B5) (CAT#: VS3-FY716)

Name: Recombinant Mouse Anti-ICAM1 Antibody (clone 1G3-9D5-6B5)
SKU: VS3-FY716
Rating: 5 (1 reviews)

This product is a recombinant mouse antibody that recognizes ICAM1. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 1G3-9D5-6B5 is specific for human ICAM1. The antigen is synthetic peptide derived from human cd54.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC-P

Figure 1. Recombinant Mouse Anti-ICAM1 Antibody (clone 1G3-9D5-6B5) in IHC-P.

Figure 1. Recombinant Mouse Anti-ICAM1 Antibody (clone 1G3-9D5-6B5) in IHC-P.

Immunohistochemical analysis of paraffin-embedded tonsil using ICAM1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg

Figure 3 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg

Figure 4 IF staining of human cell line GAMG
(Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg

Normal Tissue

Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg

Figure 6 Lung
(Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg

Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg

Figure 8 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg

Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg

RNA Expression

Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide derived from human CD54.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human ICAM1

Species Reactivity
Human

Clone
1G3-9D5-6B5

Applications
Immunohistochemistry-Paraffin

Conjugate
Unconjugated

Product Property

Purification
Affinity Chromatography

Purity
>95% as determined by SDS-PAGE

Buffer
PBS, pH 7.3, 50% glycerol, 0.5% BSA

Preservative
0.02% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Immunohistochemistry-Paraffin: 1/100-1/500

Target

Alternative Names
Intercellular Adhesion Molecule 1

Gene ID
3383

UniProt ID
P05362

Cellular Localization
Membrane

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

Isotype Control

Related Resources

Related Diseases
Related Signaling Pathways

Rheumatoid Arthritis

AGE-RAGE Signaling Pathway in Diabetic Complications

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
TAB-241	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (TAB-241)	WB, IF, IP, Neut, FuncS, ELISA, FC	Fab' - G2a
TAB-H26	Anti-Human ICAM1 Recombinant Antibody (TAB-H26)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG2a
TAB-124CT	Anti-Human ICAM-1 Recombinant Antibody (m1A6)	ELISA
TAB-124CT-S(P)	Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (m1A6)	ELISA
TAB-124CT-F(E)	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (m1A6)	ELISA

CAT	Product Name	Application	Type
TAB-121CT	Anti-Human ICAM-1 Recombinant Antibody (MD-2)	ELISA, FC	Human antibody
TAB-122CT	Anti-Human ICAM-1 Recombinant Antibody (R6-5-D6)	ELISA, FC	Human antibody
TAB-121CT-F(E)	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (MD-2)	ELISA, FC	Human antibody
TAB-122CT-F(E)	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (R6-5-D6)	ELISA, FC	Human antibody
TAB-125CT-F(E)	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (ICM 10064)	Inhibit, ELISA, WB	Human antibody

CAT	Product Name	Application	Type
TAB-123CT	Anti-Human ICAM-1 Recombinant Antibody (h1A6)	ELISA	Humanized antibody
TAB-123CT-S(P)	Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (h1A6)	ELISA	Humanized antibody
TAB-123CT-F(E)	Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (h1A6)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
NEUT-923CQ	Mouse Anti-ICAM1 Recombinant Antibody (clone CBL236)	Neut, IF, FC, WB, IHC	Mouse IgG1
NEUT-925CQ	Mouse Anti-ICAM1 Recombinant Antibody (NEUT-925CQ)	FC, FuncS, IHC, Neut, WB	Mouse IgG1, κ
NEUT-926CQ	Mouse Anti-ICAM1 Recombinant Antibody (VMC182)	FC, IF, IHC, ICC, Neut	Mouse IgG2b
NEUT-927CQ	Mouse Anti-ICAM1 Recombinant Antibody (NEUT-927CQ)	FC, IF, IHC, Neut, WB, ELISA	Mouse IgG1
NEUT-930CQ	Rat Anti-Icam1 Recombinant Antibody (NEUT-930CQ)	FC, FuncS, IHC-Fr, Neut, IP	Rat IgG2a, κ

CAT	Product Name	Application	Type
NEUT-924CQ	Mouse Anti-ICAM1 Recombinant Antibody (clone HCD54)	FC, IF, Block	Mouse IgG1, κ
NEUT-928CQ	Mouse Anti-ICAM1 Recombinant Antibody (VMC183)	Block, FC, IHC, IP	Mouse IgG1
NEUT-929CQ	Mouse Anti-ICAM1 Recombinant Antibody (NEUT-929CQ)	Block, WB, IP, ICC	Mouse IgG1

CAT	Product Name	Application	Type
MOR-1723	Rabbit Anti-ICAM1 Recombinant Antibody (clone DS1723AB), Biotin	IHC-Fr, IP, WB, ELISA	Rabbit IgG
MOR-1724	Rabbit Anti-Icam1 Recombinant Antibody (clone DS1724AB)	ELISA	Rabbit IgG
MOR-4598	Rabbit Anti-ICAM1 Recombinant Antibody (clone TH111DS)	WB, FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0053-YC-S(P)	Human Anti-ICAM1 Recombinant Antibody scFv Fragment (HPAB-0053-YC-S(P))	FC, IP	Human scFv
HPAB-662-FY-S(P)	Human Anti-ICAM1 Recombinant Antibody scFv Fragment (HPAB-662-FY-S(P))	Inhib	Human scFv
HPAB-1728-FY-F(E)	Human Anti-ICAM1 Recombinant Antibody (clone LAC1555); scFv Fragment	ELISA	Human scFv
HPAB-1729-FY-F(E)	Human Anti-ICAM1 Recombinant Antibody (clone LAC3040); scFv Fragment	ELISA	Human scFv
HPAB-1730-FY-F(E)	Human Anti-ICAM1 Recombinant Antibody (clone LAC3041); scFv Fragment	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0053-YC-F(E)	Human Anti-ICAM1 Recombinant Antibody Fab Fragment (HPAB-0053-YC-F(E))	FC, IP	Human Fab
HPAB-0859-CN-F(E)	Human Anti-ICAM1 Recombinant Antibody (clone BI-AB); Fab Fragment	ELISA, FC, Neut	Human Fab
HPAB-1728-FY-S(P)	Human Anti-ICAM1 Recombinant Antibody (clone LAC1555); Fab Fragment	ELISA	Human Fab
HPAB-1729-FY-S(P)	Human Anti-ICAM1 Recombinant Antibody (clone LAC3040); Fab Fragment	ELISA	Human Fab
HPAB-1730-FY-S(P)	Human Anti-ICAM1 Recombinant Antibody (clone LAC3041); Fab Fragment	ELISA	Human Fab

CAT	Product Name	Application	Type
ZG-0122J	Mouse Anti-ICAM1 Recombinant Antibody (ZG-0122J)	IHC-P, IF	Mouse IgG
ZG-0123J	Mouse Anti-ICAM1 Recombinant Antibody (ZG-0123J)	IHC-P, IF	Mouse IgG
ZG-0785F	Mouse Anti-ICAM1 Recombinant Antibody (clone D3.6)	FC	Mouse IgG2b
VS3-XY835	Mouse Anti-ICAM1 Recombinant Antibody, FITC (clone 4A15.2)	FC, IF	Mouse IgG1
VS3-XY836	Mouse Anti-ICAM1 Recombinant Antibody, PE (clone 4A15.2)	FC, IF	Mouse IgG1

CAT	Product Name	Application	Type
VS-0424-XY141	AbPlus™ Anti-ICAM1 Magnetic Beads (15.2)	IP, Protein Purification
VS-0724-YC1353	AbPlus™ Anti-Icam1 Magnetic Beads (VS-0724-YC1353)	IP, Protein Purification