Recombinant Rabbit Anti-ICAM1 Antibody (clone R09-7A9)

Name: Recombinant Rabbit Anti-ICAM1 Antibody (clone R09-7A9)
SKU: VS3-FY717
Rating: 5 (1 reviews)

CAT#: VS3-FY717

This product is a recombinant rabbit antibody that recognizes ICAM1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R09-7A9 is specific for human ICAM1. The antigen is human icam1 recombinant protein.

Gene Expression Tested Data

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg

Normal Tissue Figure 6 Lung

Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1

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Figure 1. Recombinant Rabbit Anti-ICAM1 Antibody (clone R09-7A9) in WB.

Western Blot analysis of ICAM1 in K562, Hela lysates using ICAM1 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-ICAM1 Antibody (clone R09-7A9) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ICAM1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

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Specifications

Immunogen
Human ICAM1 recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human ICAM1

Species Reactivity
Human

Clone
R09-7A9

Applications
Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 58 kDa; Observed MW: 89,92 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20

Target

Alternative Names
Intercellular Adhesion Molecule 1

Gene ID
3383

UniProt ID
P05362

Cellular Localization
Membrane

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY717.pdf

MSDS

MSDS - VS3-FY717.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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