Recombinant Rabbit Anti-IGFBP7 Antibody (clone R04-5D9)
CAT#: VS3-FY741
This product is a recombinant rabbit antibody that recognizes IGFBP7. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R04-5D9 is specific for human, mouse, rat IGFBP7. The antigen is synthetic peptide of human igfbp7.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Not detected
Intensity:Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_4.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, hTERT-RPE1, RPTEC TERT1, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163453-IGFBP7
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WB
Figure 1. Recombinant Rabbit Anti-IGFBP7 Antibody (clone R04-5D9) in WB.
Western Blot analysis of IGFBP7 in K562, Hela lysates using IGFBP7 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-IGFBP7 Antibody (clone R04-5D9) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using an IGFBP7 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human IGFBP7.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat IGFBP7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-5D9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 29 kDa; Observed MW: 29 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-546 | Recombinant Anti-human IGFBP7 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-546 | Recombinant Human Anti-human IGFBP7 Antibody | WB, IHC, FuncS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-546-F(E) | Recombinant Anti-human IGFBP7 Antibody Fab Fragment | WB, FACS, FuncS | Fab |
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| HPAB-0134-CN-F(E) | Mouse Anti-IGFBP7 Recombinant Antibody; Fab Fragment (HPAB-0134-CN-F(E)) | ELISA | Mouse Fab |
| HPAB-0135-CN-F(E) | Mouse Anti-IGFBP7 Recombinant Antibody; Fab Fragment (HPAB-0135-CN-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-546-S(P) | Recombinant Anti-human IGFBP7 Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| MHH-546-S(P) | Recombinant Human Anti-human IGFBP7 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| HPAB-0134-CN-S(P) | Mouse Anti-IGFBP7 Recombinant Antibody; scFv Fragment (HPAB-0134-CN-S(P)) | ELISA | Mouse scFv |
| HPAB-0135-CN-S(P) | Mouse Anti-IGFBP7 Recombinant Antibody; scFv Fragment (HPAB-0135-CN-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-097ZJ | Llama Anti-IGFBP7 Recombinant Antibody (TAB-097ZJ) | IHC, ELISA | Llama VHH |
| TAB-098ZJ | Llama Anti-IGFBP7 Recombinant Antibody (TAB-098ZJ) | ELISA, IHC, IF, FuncS | Llama VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1762 | Rabbit Anti-IGFBP7 Recombinant Antibody (clone DS1762AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC943 | AbPlus™ Anti-IGFBP7 Magnetic Beads (VS-0724-YC943) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1109 | Anti-IGFBP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3449 | Anti-Mouse IGFBP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3448 | Anti-Human IGFBP7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3450 | Anti-Rat IGFBP7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC158 | Recombinant Anti-IGFBP7 Eliminating Antibody, pH-Sensitive (VS-0625-YC158) | Antigen-Sweeping In Vivo. | Mouse IgG1 kappa |
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