Recombinant Rabbit Anti-ITGB1 Antibody (clone R06-1H5)
CAT#: VS3-FY775
This product is a recombinant rabbit antibody that recognizes ITGB1. This antibody has been reported for use in Western Blot. The clone R06-1H5 is specific for human, mouse, rat ITGB1. The antigen is human integrin β1 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69003/165766_B_5_7_selected.jpg
Normal Tissue
Figure 3 Immunofluorescent staining of human cell line BJ
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_A_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150093-ITGB1
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WB
Figure 1. Recombinant Rabbit Anti-ITGB1 Antibody (clone R06-1H5) in WB.
Western Blot analysis of integrin beta 1 in C6, 3T3, Hela lysates using integrin beta 1 antibody.
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Specifications
- Immunogen
- Human Integrin β1 Recombinant Protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat ITGB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-1H5
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 88 kDa; Observed MW: 120-160 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Integrin Subunit Beta 1
- Gene ID
- 3688
- UniProt ID
- P05556
- Sequence Similarities
- Belongs to the integrin beta chain family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Endosome, Membrane
- Post Translation Modifications
- The cysteine residues are involved in intrachain disulfide bonds.
- Protein Refseq
- NP_002202.2; NP_391988.1; NP_596867.1
- Function
- Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity).
Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099).
ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).
ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| NS-047CN | Human Anti-ITGB1 Recombinant Antibody | ELISA, WB, IHC | Human IgG |
| CAT | Product Name | Application | Type |
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| NS-047CN-F(E) | Human Anti-ITGB1 Recombinant Antibody; Fab Fragment | ELISA, WB, IHC | Human Fab |
| HPAB-0253CQ-F(E) | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21); Fab Fragment | FC | Mouse Fab |
| HPAB-0254CQ-F(E) | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2); Fab Fragment | ELISA, FC, WB, ICC, Block, IP, IHC | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-S(P) | Human Anti-ITGB1 Recombinant Antibody; scFv Fragment | ELISA, WB, IHC | Human scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0254CQ | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2) | ELISA, FC, WB, ICC, Block, IP, IF, IHC | Rat IgG1, κ |
| MRO-1978-CN | Rabbit Anti-ITGB1 Polyclonal Antibody (MRO-1978-CN) | WB, IHC, FC | Rabbit IgG |
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