Recombinant Rabbit Anti-PRKAA1 (phospho Ser496) Antibody (clone R01-3B7)
CAT#: VS3-FY1183
This product is a recombinant rabbit antibody that recognizes PRKAA1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-3B7 is specific for human PRKAA1. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser496 of human ampk alpha 1.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-PRKAA1 (phospho Ser496) Antibody (clone R01-3B7) in WB.
Western Blot analysis of AMPK alpha 1 (Phospho-Ser496) in Ramos lysates using Phospho-AMPK alpha 1 (Phospho-Ser496) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser496 of human AMPK alpha 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRKAA1
- Species Reactivity
- Human
- Clone
- R01-3B7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 64 kDa; Observed MW: 64 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1
- Gene ID
- 5562
- UniProt ID
- Q13131
- Cellular Localization
- Cytoplasm, Nucleus
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PRKAA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2844 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) | ELISA, IHC-P, WB | IgG |
| MOR-4672 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS) | WB, IF, ICC | IgG |
| MOR-0064-FY | Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0089-CN | Recombinant Mouse Anti-PRKAA1 Monoclonal Antibody (B1-E10) | WB, IF, IHC | Mouse IgG1 |
| MRO-0090-CN | Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (CBACN-025) | WB, IF, IP, FC | Rabbit IgG |
| ZG-0285F | Mouse Anti-PRKAA1 Recombinant Antibody (ZG-0285F) | WB | Mouse IgG |
| VS3-XY1296 | Mouse Anti-PRKAA1 Recombinant Antibody (clone B1-E10) | WB, ICC, IHC | Mouse IgG1 |
| VS7-0425-WR682 | Mouse Anti-PRKAA1 Monoclonal Antibody (clone 2B7) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY215 | AbPlus™ Anti-PRKAA1 Magnetic Beads (CBACN-025) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC928 | CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody (VS13-YC928) | WB, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1741 | Anti-PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5671 | Anti-Mouse PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5670 | Anti-Human PRKAA1 Immunohistochemistry Kit | IHC |
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