Recombinant Rabbit Anti-RELA Antibody (clone R09-3H2)
CAT#: VS3-FY1265
This product is a recombinant rabbit antibody that recognizes RELA. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R09-3H2 is specific for human, mouse RELA. The antigen is synthetic peptide of human nf-kb p65.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
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WB
Figure 1. Recombinant Rabbit Anti-RELA Antibody (clone R09-3H2) in WB.
Western Blot analysis of NF-KB p65 in HeLa lysates using NF-KB p65 Antibody.
WB
Figure 2. Recombinant Rabbit Anti-RELA Antibody (clone R09-3H2) in WB.
Western Blot analysis of NF-KB p65 in K562, 3T3, Hela lysates using NF-KB p65 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human NF-kB p65.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse RELA
- Species Reactivity
- Human, Mouse
- Clone
- R09-3H2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 60 kDa; Observed MW: 65 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; NFKB3; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
- Gene ID
- 5970
- UniProt ID
- Q04206
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CITATIONS
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RELA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(G) | Recombinant Anti-human RELA Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
| IAB-B027(T) | Recombinant Anti-human RELA Intrabody [Tat] | FC, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0722MZ | Chicken Anti-Nuclear factor NFκB, p65 subunit (RELA) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3043 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (DS3043AB) | FC, IP, WB | IgG |
| MOR-4654 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS) | WB, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1092-CN | Recombinant Rabbit Anti-RELA Monoclonal Antibody (CBACN-401) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2072-CN | Rabbit Anti-RELA Polyclonal Antibody (MRO-2072-CN) | WB, IHC, FC | Rabbit IgG |
| MRO-2349-CN | Recombinant Rabbit Anti-RELA (phosphorylated Ser529) Monoclonal Antibody (SP07-00) | WB, IP, FC | Rabbit IgG |
| MOB-0143F | Mouse Anti-RELA Recombinant Antibody (clone 14H2) | WB, IHC-P, IF, IP | Mouse IgG |
| MOB-0144F | Mouse Anti-RELA Recombinant Antibody (clone 5G6) | WB, IHC-P, IF, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY223 | AbPlus™ Anti-RELA Magnetic Beads (CBACN-401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY6 | Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6) | WB, IHC-P, IF, IP | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6073 | Anti-Mouse RELA Immunohistochemistry Kit | IHC |
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