Recombinant Rabbit Anti-S100A4 Antibody (clone R01-7H1)
CAT#: VS3-FY1296
This product is a recombinant rabbit antibody that recognizes S100A4. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-7H1 is specific for human S100A4. The antigen is synthetic peptide of human s100a4.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Normal Tissue
Figure 2 Lung.
High expression in lung
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
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WB
Figure 1. Recombinant Rabbit Anti-S100A4 Antibody (clone R01-7H1) in WB.
Western Blot analysis of S100A4 in HeLa lysates using S100A4 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-S100A4 Antibody (clone R01-7H1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using S100A4 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human S100A4.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A4
- Species Reactivity
- Human
- Clone
- R01-7H1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 12 kDa; Observed MW: 12 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- S100 Calcium Binding Protein A4; S100 Calcium-Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Placental Calcium-Binding Protein; Fibroblast-Specific Protein-1; Protein Mts1; MTS1; CAPL; S100 Calcium Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Leukemia Multidrug Resistance Associated Protein; Malignant Transformation Suppression 1
- Gene ID
- 6275
- UniProt ID
- P26447
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Protein Refseq
- NP_002952.1; NP_062427.1
- Function
- Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770).
Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441).
Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity).
Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197).
Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity).
In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:30713770, PubMed:26654597).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3568z | Mouse Anti-S100A4 Recombinant Antibody (clone 24D3) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
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| VS3-WK1924 | Recombinant Rabbit Anti-Mouse S100A4 Capture Antibody (clone R82-5X-2) | ELISA | Rabbit IgG |
| VS3-WK1925 | Recombinant Rabbit Anti-Mouse S100A4 Detection Antibody (clone R55-3Y-2) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0136CL | Mouse Anti-S100A4 Recombinant Antibody (TAB-0136CL) | WB, IF, Block | Mouse IgG |
| TAB-0135CL-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-0135CL-F(E)) | WB, ELISA, BI, Block, Inhib | Mouse Fab |
| TAB-0136CL-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-0136CL-F(E)) | WB, ELISA, BI, Block, Inhib | Mouse Fab |
| TAB-379CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-379CT) | WB, IHC, Block | Mouse IgG2a |
| TAB-380CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-380CT) | WB, IHC, Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Single Domain Antibody (TAB-377CT), Research Grade Biosimilar | WB, ELISA | Single domain antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1028 | CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody (VS13-YC1028) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1992 | Anti-S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6304 | Anti-Mouse S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6303 | Anti-Human S100A4 Immunohistochemistry Kit | IHC |
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