Recombinant Human Anti-SEMA4D Antibody (clone 5D12)
CAT#: VS4-CJ110
This human anti-SEMA4D antibody (clone 5D12) is a product that recognizes human, mouse SEMA4D. The immunogen of this antibody is recombinant human SEMA4D protein. The mAb has been tested for use in ELISA, Flow Cytometry.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23277/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15662/37983_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HDLM-2, HMC-1, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187764-SEMA4D
❮
❯
Specifications
- Immunogen
- Recombinant Human SEMA4D protein
- Host Species
- Human
- Type
- Human IgG4 (S228P)
- Specificity
- Human, Mouse SEMA4D
- Species Reactivity
- Human, Mouse
- Clone
- 5D12
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.01M PBS, pH 7.4.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Flow Cytometry.
Target
- Alternative Names
- A8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
- Gene ID
- 10507
- UniProt ID
- Q92854
- Long Name
- Semaphorin 4D
- Sequence Similarities
- Belongs to the semaphorin family.
- Cellular Localization
- Cell membrane, Membrane
- Protein Refseq
- NP_001135759.1; NP_006369.3
- Function
- Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282).
Regulates GABAergic synapse development (By similarity).
Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity).
Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569).
Promotes the migration of cerebellar granule cells (PubMed:16055703).
Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214).
Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS4-CJ110. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SEMA4D"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-429CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-429CT) | WB | Humanized IgG |
| TAB-430CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-430CT) | WB | Humanized IgG |
| TAB-429CT-S(P) | Humanized Anti-SEMA4D Recombinant Antibody; scFv Fragment (TAB-429CT-S(P)) | WB, ELISA, FC, BL | Humanized scFv |
| TAB-429CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-429CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
| TAB-430CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-430CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3206 | Hi-Affi™ Recombinant Rabbit Anti-SEMA4D Monoclonal Antibody (DS3206AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0049-WJ | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-0049-WJ) | FC, ELISA, IHC, FuncS, Inhib | Mouse IgG1 |
| HPAB-AP440-YC | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-AP440-YC) | ELISA, Block, Neut, FuncS | Mouse IgG2b |
| HPAB-AP442-YC | Mouse Anti-SEMA4D Recombinant Antibody (HPAB-AP442-YC) | ELISA, Block, Neut, FuncS | Mouse IgG2b |
| HPAB-AP443-YC | Human Anti-SEMA4D Recombinant Antibody (HPAB-AP443-YC) | ELISA, FC, Block | Human IgG1 |
| HPAB-AP444-YC | Human Anti-SEMA4D Recombinant Antibody (HPAB-AP444-YC) | ELISA, FC, Block | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0049-WJ-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-0049-WJ-S(P)) | FC, ELISA | Mouse scFv |
| HPAB-AP440-YC-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-AP440-YC-S(P)) | ELISA, Block, Neut, FuncS | Mouse scFv |
| HPAB-AP442-YC-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-AP442-YC-S(P)) | ELISA, Block, Neut, FuncS | Mouse scFv |
| HPAB-0934WJ-S(P) | Mouse Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-0934WJ-S(P)) | Neut | Mouse scFv |
| HPAB-0935WJ-S(P) | Human Anti-SEMA4D Recombinant Antibody; scFv Fragment (HPAB-0935WJ-S(P)) | Neut, ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0049-WJ-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0049-WJ-F(E)) | FC, ELISA | Mouse Fab |
| HPAB-AP440-YC-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-AP440-YC-F(E)) | ELISA, Block, Neut, FuncS | Mouse Fab |
| HPAB-AP442-YC-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-AP442-YC-F(E)) | ELISA, Block, Neut, FuncS | Mouse Fab |
| HPAB-0934WJ-F(E) | Mouse Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0934WJ-F(E)) | Neut | Mouse Fab |
| HPAB-0935WJ-F(E) | Human Anti-SEMA4D Recombinant Antibody; Fab Fragment (HPAB-0935WJ-F(E)) | Neut, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY70 | Mouse Anti-NHP SEMA4D Recombinant Antibody (clone A8) | IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY197 | CytoStream™ Rat Anti-SEMA4D Recombinant Antibody (clone BMA-12) | FC | Rat IgG2a, lambda |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC533 | Recombinant Anti-SEMA4D Vesicular Antibody, EV Displayed (VS-0425-YC533) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6444 | Anti-SEMA4D Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-2.png)
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA, FC, IHC, Neut
-2.png)
Application: ELISA
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
