Recombinant Rabbit Anti-Sirt1 Antibody (clone R06-4K9)
CAT#: VS3-FY1338
This product is a recombinant rabbit antibody that recognizes Sirt1. This antibody has been reported for use in Western Blot. The clone R06-4K9 is specific for human, mouse, rat Sirt1. The antigen is mouse sirt1 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/43_F3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52351/826_A6_1_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_A_4_6.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_B_6_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096717-SIRT1
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WB
Figure 1. Recombinant Rabbit Anti-Sirt1 Antibody (clone R06-4K9) in WB.
Western Blot analysis of SIRT1 in K562, C6, 3T3, Hela lysates using SIRT1 Antibody.
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Specifications
- Immunogen
- mouse SIRT1 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat Sirt1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-4K9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 80 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Sirtuin 1; Regulatory Protein SIR2 Homolog 1; SIR2-Like Protein 1; SIR2L1; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 1; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. Cerevisiae); NAD-Dependent Protein Deacetylase Sirtuin-1
- Gene ID
- 23411
- UniProt ID
- Q96EB6
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SIRT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0777MZ | Chicken Anti-SIRT1 Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3267 | Hi-Affi™ Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (DS3267AB) | IP, WB | IgG |
| MOR-0043-FY | Rabbit Anti-SIRT1 Recombinant Antibody (clone AFY0014) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2188-CN | Rabbit Anti-SIRT1 Polyclonal Antibody (MRO-2188-CN) | WB, IHC, FC | Rabbit IgG |
| ZG-0071U | Rabbit Anti-SIRT1 Recombinant Antibody (clone 9E9) | ELISA, IHC, IF | Rabbit IgG |
| VS3-WK2136 | Rabbit Anti-SIRT1 (pS47) Recombinant Antibody (clone R04-7M2) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2148 | Rabbit Anti-SIRT1 (pT530) Recombinant Antibody (clone R06-2U8) | WB | Rabbit IgG |
| VS7-0425-WR854 | Mouse Anti-SIRT1 Monoclonal Antibody (VS7-0425-WR854) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY235 | AbPlus™ Anti-SIRT1 Magnetic Beads (CBACN-504) | IP, Protein Purification | |
| VS-0724-YC526 | AbPlus™ Anti-SIRT1 Magnetic Beads (VS-0724-YC526) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY480 | Mouse Anti-NHP SIRT1 Recombinant Antibody (clone 1F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2070 | Anti-SIRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6601 | Anti-Human SIRT1 Immunohistochemistry Kit | IHC |
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