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SMAD5
Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5)

Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) (CAT#: VS3-FY1360)

Name: Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5)
SKU: VS3-FY1360
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes SMAD5. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-2A5 is specific for human, rat SMAD5. The antigen is synthetic peptide of human smad5.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) in WB.

Figure 1. Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) in WB.

Western Blot analysis of Smad5 in C6, Hela lysates using Smad5 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) in IHC-P.

Figure 2. Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SMAD5 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg

Normal Tissue

Figure 2 IHC staining of human uterus, post-menopause
(Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg

Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg

Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg

Figure 6 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg

Figure 7 Endometrium
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human SMAD5.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Rat SMAD5

Species Reactivity
Human, Rat

Clone
R07-2A5

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 52 kDa; Observed MW: 60 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
SMAD Family Member 5; MADH5; JV5-1; MAD, Mothers Against Decapentaplegic Homolog 5 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 5; SMAD, Mothers Against DPP Homolog 5 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 5; Mothers Against Decapentaplegic Homolog 5; SMAD, Mothers Against DPP Homolog 5

Gene ID
4090

UniProt ID
Q99717

Cellular Localization
Cytoplasm, Nucleus

Secondary Antibody

Isotype Control

Related Resources

Related Signaling Pathways

TGF-β Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "SMAD5"

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IgG Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-1798MZ	Recombinant Mouse Anti-Human SMAD5 Antibody	ICC/IF, WB	Mouse antibody
ZG-0568U	Rabbit Anti-Phospho-SMAD5 (S463+S465) Recombinant Antibody (clone 2G11)	ELISA, IHC	Rabbit IgG
ZG-0569U	Rabbit Anti-Phospho-SMAD5 (S463/S465) Recombinant Antibody (clone 3A9)	ELISA, WB	Rabbit IgG
VS3-QX1029	Mouse Anti-SMAD5 Recombinant Antibody (clone 4B10-B10-B6)	WB, ICC, FC	Mouse IgG1
VS3-XY1435	Mouse Anti-SMAD5 Recombinant Antibody (clone 3H9)	ELISA, WB, FC, IHC, ICC	Mouse IgG1

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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