Recombinant Rabbit Anti-Stat5A Antibody (clone R09-5F2)
CAT#: VS3-FY1401
This product is a recombinant rabbit antibody that recognizes Stat5A. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R09-5F2 is specific for human, mouse, rat Stat5A. The antigen is mouse stat5a recombinant protein.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42128/ihc_selected.jpg
Normal Tissue
Figure 2 Soft tissue
Peripheral nerve
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_B_2_8.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3860/11333_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HDLM-2, HEL, HMC-1, K-562, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126561-STAT5A/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-Stat5A Antibody (clone R09-5F2) in WB.
Western Blot analysis of STAT5a/b in K562, C6, 3T3, Hela lysates using STAT5 Antibody.
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Specifications
- Immunogen
- mouse STAT5A recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat Stat5A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-5F2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 91 kDa; Observed MW: 91 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- MGF; STAT5
- Gene ID
- 20850
- UniProt ID
- P42229
- Sequence Similarities
- Belongs to the transcription factor STAT family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity).
Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090).
Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity).
Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).
Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3 (PubMed:14504097).
Alternatively, can be phosphorylated by JAK2 at Tyr-694 (PubMed:12529425).
ISGylated.
- Protein Refseq
- NP_001275647.1; NP_001275648.1; NP_001275649.1
- Function
- Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
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Related Signaling Pathways
ErbB Signaling Pathway
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "STAT5A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2044z | Mouse Anti-STAT5A Recombinant Antibody (clone 30F6) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1441-CN | Recombinant Rabbit Anti-STAT5A Monoclonal Antibody (CBACN-532) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX1069 | Mouse Anti-STAT5A Recombinant Antibody (clone 5C10-D3-D10) | WB | Mouse IgG2b |
| VS3-CJ947 | Rabbit Anti-STAT5A Recombinant Antibody (VS3-CJ947) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ948 | Rabbit Anti-STAT5A (phospho Y694) Recombinant Antibody (VS3-CJ948) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3429 | Hi-Affi™ Recombinant Rabbit Anti-STAT5A Monoclonal Antibody (DS3429AB) | WB, IHC-P, ICC, IF, FC, IP | IgG |
| MOR-4712 | Hi-Affi™ Recombinant Rabbit Anti-STAT5A Monoclonal Antibody (TH226DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| MOR-4743 | Hi-Affi™ Recombinant Rabbit Anti-STAT5A Monoclonal Antibody (TH257DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC470 | AbPlus™ Anti-STAT5a Magnetic Beads (VS-0724-YC470) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2197 | Anti-STAT5A Immunohistochemistry Kit | IHC | |
| VS-0525-XY6957 | Anti-Mouse STAT5A Immunohistochemistry Kit | IHC | |
| VS-0525-XY6956 | Anti-Human STAT5A Immunohistochemistry Kit | IHC | |
| VS-0525-XY6958 | Anti-Rat STAT5A Immunohistochemistry Kit | IHC |
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