Recombinant Mouse Anti-ZAP70 Antibody (clone 6C9-6H9-7E7)
CAT#: VS3-FY1574
This product is a recombinant mouse antibody that recognizes ZAP70. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 6C9-6H9-7E7 is specific for human ZAP70. The antigen is synthetic peptide derived from human zap-70.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_8_5.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_B_5_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3134/8986_A_7_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115085-ZAP70/subcellular
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IHC-P
Figure 1. Recombinant Mouse Anti-ZAP70 Antibody (clone 6C9-6H9-7E7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using ZAP70 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide derived from human ZAP-70.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ZAP70
- Species Reactivity
- Human
- Clone
- 6C9-6H9-7E7
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22.
Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
- Protein Refseq
- NP_001070.2; NP_997402.1
- Function
- Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
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Related Signaling Pathways
BCR Signaling Pathway
TCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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- Product Name
Isotype Control
- CAT
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Reconstitution Buffer
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Recommended Dilution Buffer
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| CAT | Product Name | Application | Type |
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| MOR-3911 | Rabbit Anti-ZAP70 Recombinant Antibody (clone DS3911AB) | WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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