Mouse Anti-B2M Recombinant Antibody (clone YTH470.5)
CAT#: NEUT-034CQ
This antibody is a mouse monoclonal antibody that binds specifically to B2BM, and it can neutralize the bioactivity of B2BM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
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Specifications
- Immunogen
- Human peripheral mononuclear cells enriched for T cells.
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Recognizes human beta2-microglobulin, a component of MHC class I molecules
- Species Reactivity
- Human
- Clone
- YTH470.5
- Applications
- FC, Block
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Works in Multiple Formats
This antibody works well across various experimental formats, offering versatility which is vital for multi-faceted research projects.
Positive Lab Experience
The antibody offered consistent results across repeated experiments. It's become a go-to tool in our lab.
Reliable Readouts
We've repeatedly used this antibody in our experiments and it consistently provides reliable data.
Q&As
-
How is the antibody purified?
A: It is typically purified using Protein A or G affinity chromatography, ensuring high purity and low contaminants.
-
What is the shelf life of this antibody when stored properly?
A: The shelf life is generally one year from the date of receipt, provided it is stored under recommended conditions.
-
Does the antibody require any special handling procedures?
A: Normal laboratory handling procedures should be followed, but minimize freeze-thaw cycles to maintain antibody integrity.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| HPAB-0130-FY-S(P) | Human Anti-B2M Recombinant Antibody (clone B2M-1D8); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0131-FY-S(P) | Human Anti-B2M Recombinant Antibody (clone B2M-2F6); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0350-FY-S(P) | Mouse Anti-B2M Recombinant Antibody (clone FY-007); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-076 | Recombinant Anti-B2M VHH Single Domain Antibody (PNBL-076) | FACS, BL | Llama VHH |
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| CAT | Product Name | Application | Type |
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| BRD-1010MZ | Chicken Anti-beta-2 Microglobulin Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-0312 | Hi-Affi™ Rabbit Anti-B2M Recombinant Antibody (clone DS312AB) | ELISA | Rabbit IgG |
| MOR-3971 | Hi-Affi™ Rabbit Anti-B2M Recombinant Antibody (clone SI31DS) | IHC-P | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1304 | AbPlus™ Anti-B2M Magnetic Beads (VS-0724-YC1304) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY51 | Mouse Anti-NHP B2M Recombinant Antibody (clone C21.48A1) | WB, IF, FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY218 | Anti-B2M Immunohistochemistry Kit | IHC | |
| VS-0525-XY671 | Anti-Human B2M Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC27 | Recombinant Anti-B2M Eliminating Antibody, pH-Sensitive (YTH 470.5 var.) | Antigen-Sweeping In Vivo. | Mouse IgG1 |
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