Recombinant Anti-Human B2M Antibody Fab Fragment
CAT#: MOB-1431z-F(E)
Recombinant Mouse Antibody Fab Fragment is closely related to binding with Human B2M, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
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Specifications
- Immunogen
- Human Beta-2-Microglobulin
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Human Beta-2-Microglobulin
- Species Reactivity
- Human
- Applications
- Used for immunoassaytechniques such as: Neutralization; Western blot; Functional Study
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- ≤6 months at 4°C; ≥6 months at -20°C.
Target
REVIEWS AND Q&AS
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Customer Review
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Great Fab Fragment
I have been utilizing this recombinant anti-B2M antibody Fab fragment in western blot and neutralization assays. Its high purity (>95% by SDS-PAGE) and consistent binding to human B2M provide robust performance in dissecting MHC class I related molecular interactions in my lab.
Consistent Fab
The mouse-derived Fab fragment against human B2M works reliably in multiple immunoassays. Its excellent purity, as validated by SDS-PAGE, ensures clear and reproducible results when examining the role of Beta-2-Microglobulin in MHC I presentation. I am very satisfied with its performance.
Q&As
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Can the Fab fragment be used in western blot?
A: Yes, the recombinant anti-B2M Fab fragment has been demonstrated to work effectively in western blot and neutralization assays. Its high purity (>95% by SDS-PAGE) and specific binding to human B2M make it an ideal reagent for studying protein-protein interactions in vitro.
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What attributes support its use in functional studies?
A: The Fab fragment's excellent purity and specific reactivity to human Beta-2-Microglobulin ensure that it delivers reproducible results in immunoassays such as western blot and neutralization tests. Its recombinant production guarantees consistency across various functional research protocols.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "B2M"
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|---|---|---|---|
| MOB-1431z | Mouse Anti-B2M Recombinant Antibody (MOB-1431z) | Block, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431z-S(P) | Recombinant Anti-Human B2M Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-076 | Recombinant Anti-B2M VHH Single Domain Antibody (PNBL-076) | FACS, BL | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0078MZ | Mouse Anti-B2M Recombinant Antibody (clone C2G8) | FC, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1592MZ | Mouse Anti-B2M Recombinant Antibody (clone IZC443-02) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1010MZ | Chicken Anti-beta-2 Microglobulin Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| NEUT-034CQ | Mouse Anti-B2M Recombinant Antibody (clone YTH470.5) | FC, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0312 | Hi-Affi™ Rabbit Anti-B2M Recombinant Antibody (clone DS312AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3971 | Hi-Affi™ Rabbit Anti-B2M Recombinant Antibody (clone SI31DS) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4538 | Hi-Affi™ Rabbit Anti-B2M Recombinant Antibody (clone TH46DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2379 | PE-H2-IAd/Human B2M (ACRVNHVTLSQPKIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2380 | PE-H2-IEd/Human B2M (ACRVNHVTLSQPKIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2381 | PE-DRB1*01:01/Human B2M (ACRVNHVTLSQPKIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2382 | PE-DRB5*01:01/Human B2M (ACRVNHVTLSQPKIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2383 | PE-H2-IAd/Human B2M (CYVSGFHPSDIEVDLL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-FY | Human Anti-B2M Recombinant Antibody (clone B2M-1D8) | ELISA, FC | Humanized IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0131-FY | Human Anti-B2M Recombinant Antibody (clone B2M-2F6) | ELISA, FC | Humanized IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-FY-S(P) | Human Anti-B2M Recombinant Antibody (clone B2M-1D8); scFv Fragment | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0131-FY-S(P) | Human Anti-B2M Recombinant Antibody (clone B2M-2F6); scFv Fragment | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-FY-F(E) | Human Anti-B2M Recombinant Antibody (clone B2M-1D8); Fab Fragment | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0131-FY-F(E) | Human Anti-B2M Recombinant Antibody (clone B2M-2F6); Fab Fragment | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0350-FY-S(P) | Mouse Anti-B2M Recombinant Antibody (clone FY-007); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0350-FY-F(E) | Mouse Anti-B2M Recombinant Antibody (clone FY-007); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-A1221-YJ | Camelid Anti-B2M Recombinant Antibody (HPAB-A1221-YJ) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1304 | AbPlus™ Anti-B2M Magnetic Beads (VS-0724-YC1304) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1371 | AbPlus™ Anti-B2M Magnetic Beads (VS-0724-YC1371) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY51 | Mouse Anti-NHP B2M Recombinant Antibody (clone C21.48A1) | WB, IF, FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY218 | Anti-B2M Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY671 | Anti-Human B2M Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC27 | Recombinant Anti-B2M Eliminating Antibody, pH-Sensitive (YTH 470.5 var.) | Antigen-Sweeping In Vivo. | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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