PE-H2-IAd/Human B2M (CYVSGFHPSDIEVDLL) MHC Tetramer

Name: PE-H2-IAd/Human B2M (CYVSGFHPSDIEVDLL) MHC Tetramer
SKU: MHC-LC2383
Rating: 5 (1 reviews)

CAT#: MHC-LC2383

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of human B2M of peptide CYVSGFHPSDIEVDLL covering 45-60 and H2-IAd molecule. The pMHC tetramer recognizes CD4 T cells, and can be used in the analysis of individual antigen-specific T cells.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg

Normal Tissue Figure 3 Spleen

High expression in spleen

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg

Normal Tissue Figure 4 Bone marrow

Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg

Normal Tissue Figure 6 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg

RNA Expression Figure 8 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M

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Specifications

Allele
H2-IAd

Class
Class II

MHC Species
Mouse

Antigen
B2M

Antigen Species
Human

Peptide
CYVSGFHPSDIEVDLL

Range
45-60

Conjugate
PE

Application
FCM

Target

Antigen Introduction
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Alternative Names
B2M; Beta-2-microglobulin precursor; IMD43

Gene ID
567

UniProt ID
P61769

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Datasheet

Datasheet - MHC-LC2383.pdf

MSDS

MSDS - MHC-LC2383.pdf

COA

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