Chimeric Anti-Human CD27 Recombinant Antibody (2F2) (CAT#: TAB-034LC)

Recombinant monoclonal antibody to CD27. 2F2 is a chimeric antibody (rabbit/human) that can be potentially used in the treatment of autoimmune diseases.


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Block

Figure 1 2F2 blocks CD27 downrnodulation induced by Ramos in a dose-dependent fashion.

Figure 1 2F2 blocks CD27 downrnodulation induced by Ramos in a dose-dependent fashion.

T cells were preactivated as in method. For CD27 downrnodulation, cells were incubated with (black bars) or without (hatched bar) irradiated Rarnos cells for 18 h. For cultures containing Rarnos cells, titrated concentrations of purified 2F2 rnAb were added as indicated on the vertical axis. Mean fluorescence plus SE of three independent experiments is shown.

HintZen, R. Q., Lens, S. M., Beckmann, M. P., Goodwin, R. G., Lynch, D., & Van Lier, R. A. (1994). Characterization of the human CD27 ligand, a novel member of the TNF gene family. The Journal of Immunology, 152(4), 1762-1773.

IP

Figure 2 Biochemical analysis of CD27L.

Figure 2 Biochemical analysis of CD27L.

Ramos cells were either irradiated (lanes B and C) or not (lanes D and E) and surface iodinated as described in Materials and Methods. Cells were lysed in 1% NP-40 buffer and immunoprecipitation was conducted using CLB-CD27/1 (lanes B and D) and 2F2 mAb (lanes C and E)-coated CNBr-activated Sepharose 48 beads. Beads coated with CLB-MH15 (lane A) were used as a control. Precipitates were analyzed under reducing conditions on a 5 to 15% SDS polyacrylamide gradient gel.

HintZen, R. Q., Lens, S. M., Beckmann, M. P., Goodwin, R. G., Lynch, D., & Van Lier, R. A. (1994). Characterization of the human CD27 ligand, a novel member of the TNF gene family. The Journal of Immunology, 152(4), 1762-1773.


Specifications

  • Host Species
  • Human
  • Derivation
  • Chimeric (rabbit/human)
  • Type
  • Chimeric antibody (rabbit/human)
  • Specificity
  • Human
  • Clone
  • 2F2
  • Applications
  • Block, IP
  • Related Disease
  • Autoimmune diseases

Applications

  • Application Notes
  • This antibody has been reported for use in Immunoprecipitation and Blocking. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

  • Alternative Names
  • CD27; CD27 molecule; T14; S152; Tp55; TNFRSF7; S152. LPFS2; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 2F2"

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Recombinant Antibody

Human Antibody

CAT Product Name Application Type
TAB-H74 Anti-Human CD27 Recombinant Antibody (Varlilumab) ELISA, IP, FC, FuncS, Neut, IF, ICC IgG1 - kappa

Chimeric Antibody

CAT Product Name Application Type
TAB-018LC Human Anti-CD27 Recombinant Antibody (TAB-018LC) ELISA Chimeric antibody (rat/human)
TAB-019LC Human Anti-CD27 Recombinant Antibody (TAB-019LC) ELISA Chimeric antibody (rat/human)
TAB-020LC Human Anti-CD27 Recombinant Antibody (TAB-020LC) ELISA Chimeric antibody (rat/human)
TAB-021LC Human Anti-CD27 Recombinant Antibody (TAB-021LC) ELISA Chimeric antibody (rat/human)
TAB-037LC-F(E) Anti-Human CD27 Recombinant Antibody Fab Fragment (0152) SPR, FC Chimeric antibody (rabbit/human)

Neutralizing Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0547 Hi-Affi™ Rabbit Anti-CD27 Recombinant Antibody (clone DS547AB) WB, IHC, ICC, FC Rabbit IgG

Agonistic Antibody

CAT Product Name Application Type
MOB-096LC Human Anti-CD27 Recombinant Antibody (MOB-096LC) ELISA, FuncS, WB Human IgG1, κ

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H74 Afuco™ Anti-CD27 ADCC Recombinant Antibody (Varlilumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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