Human Anti-EPHA2 Recombinant Antibody (clone 4H5) (CAT#: HPAB-0453-CN)

Figure 1 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SDS-PAGE

SDS-PAGE analysis of HPAB-0453-CN in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in SEC-HPLC

The purity of HPAB-0453-CN was 99.1491% as determined by SEC-HPLCInjection: 10 µlInjection: 10 µl

Figure 3 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in WB

Western blot analysis of HPAB-0453-CN was performed by loading EPHA2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0453-CN and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in DB

Dot Blot analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag).

HPAB-0453-CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)

Figure 5 Anti-EPHA2 Recombinant Antibody (HPAB-0453-CN) in ELISA

ELISA analysis of HPAB-0453-CN was performed by coating with EPHA2 protein (His tag). Then blocked with BSA and incubated with HPAB-0453-CN. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.