Human Anti-EPHA2 Recombinant Antibody (clone 4H5); scFv Fragment (CAT#: HPAB-0453-CN-S(P))
4H5 is an antibody which is humanized from murine antibody EA2. 4H5 exhibits approximately a 20 fold increase of affinity when compared with chimeric Fab EA2.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles, plasma membrane, the Golgi apparatus & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10464/991_D3_3_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles, plasma membrane, the Golgi apparatus & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10464/991_D3_3_selected.jpg
Figure 2 RNA cell line category: Cell line enhanced (CAPAN-2, PC-3, U-251 MG, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142627-EPHA2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142627-EPHA2
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized scFv
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- 4H5
- Applications
- ELISA, FC, IF
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Soluble EPHA2 Variant 1; Epithelial Cell Kinase; EC 2.7.10; CTRCT6; ARCC2; CTPP1; EphA2; CTPA
- Gene ID
- 1969
- UniProt ID
- P29317
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
Humanized Antibody
Mouse Antibody
MHC Tetramer for Cancer
Rabbit Monoclonal Antibody
Fab Fragment Antibody
scFv Fragment Antibody
Agonistic Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1460z | Mouse Anti-EPHA2 Recombinant Antibody (clone 31G12) | WB, ELISA, ICC, IF | Mouse IgG2b, κ |
| HPAB-0450-CN | Mouse Anti-EPHA2 Recombinant Antibody (HPAB-0450-CN) | ELISA, WB, FC, IP | Mouse IgG2a |
| HPAB-0452-CN | Human Anti-EPHA2 Recombinant Antibody (HPAB-0452-CN) | ELISA, WB, FC, IP | Humanized IgG1 |
| HPAB-0453-CN | Human Anti-EPHA2 Recombinant Antibody (clone 4H5) | ELISA, FC, IF | Humanized IgG |
| HPAB-0454-CN | Human Anti-EPHA2 Recombinant Antibody (clone 2A4) | ELISA, FC, IF, Agonist | Humanized IgG |
Customer Reviews and Q&As
Customer Review
Q&As
-
Cost-Effective
Considering the high performance and reliability, this antibody is quite cost-effective. It's worth the investment for any lab focusing on EPHA2. -
Highly Reliable Performance
The Human Anti-EPHA2 Recombinant Antibody (clone 4H5) provided consistent and reliable results in our Western blot assays. It has become a staple in our lab for EPHA2-related studies. -
Works Well With Various Cell Lines
We tested this antibody across different human cell lines. It recognized EPHA2 effectively in all of them, proving its broad applicability.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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