I'm planning to use this anti-FN1 antibody in an ELISA to detect human fibronectin. Could you confirm the host and isotype? Is this antibody known to cross-react with fibronectin from other species?

Our Anti-FN1 Recombinant Antibody (CAT: HPAB-0193-CN) is a mouse antibody with an IgG1 isotype. It is engineered for high specificity and is validated for use in ELISA. According to our current data, it has been tested for reactivity against human FN1. We do not have data regarding its cross-reactivity with other species, so this would need to be determined by the researcher.

I am looking for a monoclonal antibody against FN1. What is the advantage of using this recombinant version compared to a traditionally produced monoclonal antibody?

Our recombinant Anti-FN1 antibody offers several key advantages. Because it is produced using recombinant technology, it provides superior batch-to-batch consistency, which is crucial for long-term studies requiring reproducible results. This method also ensures a high level of purity and defined characteristics, such as its mouse IgG1 isotype. The defined sequence eliminates the risk of genetic drift that can occur in hybridoma cell lines.

Mouse Anti-FN1 Recombinant Antibody (HPAB-0193-CN)

CAT#: HPAB-0193-CN

This product is a recombinant Mouse antibody that recognizes FN1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg

Normal Tissue Figure 3 Liver

Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg

Normal Tissue Figure 4 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg

Normal Tissue Figure 5 Placenta

Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1

❮

❯

Specifications

Immunogen
Synthesized peptides based on TYSSPEDGIHELFP

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human FN1

Species Reactivity
Human, Mouse

Applications
ELISA, FC, IHC

Related Disease
Ischemic disease

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Fibronectin 1; Cold-Insoluble Globulin; Migration-Stimulating Factor; CIG; FN; GFND2; ED-B; FINC; GFND; LETS; FNZ; MSF

Gene ID
2335

UniProt ID
P02751

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for HPAB-0193-CN. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Consistent ELISA Results

This recombinant anti-FN1 antibody provided very consistent and reproducible results in our ELISA assays. The high purity meant we had very clean data with minimal background signal. The fact that it is a human monoclonal IgG1 was perfect for our experimental design, avoiding issues with anti-mouse secondary antibodies. I'm very satisfied with its performance.

Great Specificity for Human FN1

We needed a reliable antibody to detect human FN1, and this product delivered. It showed excellent specificity in our ELISA setup. The batch-to-batch consistency of a recombinant antibody is a huge plus for us, as we need our results to be comparable over time. The product performed exactly as described on the datasheet.

Q&As

I'm planning to use this anti-FN1 antibody in an ELISA to detect human fibronectin. Could you confirm the host and isotype? Is this antibody known to cross-react with fibronectin from other species?

A: Our Anti-FN1 Recombinant Antibody (CAT: HPAB-0193-CN) is a mouse antibody with an IgG1 isotype. It is engineered for high specificity and is validated for use in ELISA. According to our current data, it has been tested for reactivity against human FN1. We do not have data regarding its cross-reactivity with other species, so this would need to be determined by the researcher.
I am looking for a monoclonal antibody against FN1. What is the advantage of using this recombinant version compared to a traditionally produced monoclonal antibody?

A: Our recombinant Anti-FN1 antibody offers several key advantages. Because it is produced using recombinant technology, it provides superior batch-to-batch consistency, which is crucial for long-term studies requiring reproducible results. This method also ensures a high level of purity and defined characteristics, such as its mouse IgG1 isotype. The defined sequence eliminates the risk of genetic drift that can occur in hybridoma cell lines.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

AGE-RAGE Signaling Pathway in Diabetic Complications

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0193-CN.pdf

MSDS

MSDS - HPAB-0193-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "FN1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
TAB-193	Anti-Human Fibronectin Extra Domain-B Recombinant Antibody (Radretumab)	IF, IP, Neut, FuncS, ELISA, FC, ICC	[(scFv - heavy - kappa) - IGHE - CH4]2

CAT	Product Name	Application	Type
MOB-1294z	Mouse Anti-FN1 Recombinant Antibody (clone 6H4)	IHC, IP, WB	Mouse IgG2b, κ

CAT	Product Name	Application	Type
TAB-001CQ	Anti-Human FN1 Recombinant Antibody (MOR03255)	ELISA, Block, IHC	Human antibody

CAT	Product Name	Application	Type
TAB-002CQ	Anti-Human FN1 Recombinant Antibody (ME-4C)	ELISA, IHC	Human antibody

CAT	Product Name	Application	Type
TAB-003CQ	Anti-Human FN1 Recombinant Antibody (1C5)	ELISA, WB	Human antibody

CAT	Product Name	Application	Type
TAB-004CQ	Human Anti-FN1 Recombinant Antibody (TAB-004CQ)	ELISA, IHC, WB	Human IgG

CAT	Product Name	Application	Type
MOB-0025MZ	Recombinant Mouse Anti-Human Fibronectin Antibody (clone C715M)	ELISA	Mouse antibody

CAT	Product Name	Application	Type
MOB-1516MZ	Recombinant Mouse Anti-Human FN1 Antibody (clone 3G23)	ELISA, WB	Mouse antibody

CAT	Product Name	Application	Type
NEUT-852CQ	Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1)	ELISA, WB, IP, Block	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-853CQ	Mouse Anti-FN1 Recombinant Antibody (clone IST-9)	WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P	Mouse IgG1

CAT	Product Name	Application	Type
MOR-1326	Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB)	IHC-Fr, IHC-P, IHC-P, IP, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0194-CN	Mouse Anti-FN1 Recombinant Antibody (HPAB-0194-CN)	ELISA, FC, IHC	Mouse IgG1

CAT	Product Name	Application	Type
HPAB-0195-CN	Human Anti-FN1 Recombinant Antibody (HPAB-0195-CN)	ELISA, FC, IHC	Humanized IgG

CAT	Product Name	Application	Type
HPAB-0193-CN-S(P)	Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0193-CN-S(P))	ELISA, FC, IHC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0194-CN-S(P)	Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0194-CN-S(P))	ELISA, FC, IHC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0195-CN-S(P)	Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0195-CN-S(P))	ELISA, FC, IHC	Human scFv

CAT	Product Name	Application	Type
HPAB-0196-CN-S(P)	Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0196-CN-S(P))	ELISA, FC, IHC	Human scFv

CAT	Product Name	Application	Type
HPAB-0193-CN-F(E)	Mouse Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0193-CN-F(E))	ELISA, FC, IHC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0194-CN-F(E)	Mouse Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0194-CN-F(E))	ELISA, FC, IHC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0195-CN-F(E)	Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0195-CN-F(E))	ELISA, FC, IHC	Humanized Fab

CAT	Product Name	Application	Type
HPAB-0196-CN-F(E)	Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0196-CN-F(E))	ELISA, FC, IHC	Humanized Fab

CAT	Product Name	Application	Type
FAMAB-0105-YC-S(P)	Human Anti-FN1 Recombinant Antibody (clone ME4C); scFv Fragment	ELISA, IHC	Human scFv

CAT	Product Name	Application	Type
FAMAB-0105-YC-F(E)	Human Anti-FN1 Recombinant Antibody (clone ME4C); Fab Fragment	ELISA, IHC	Human Fab

CAT	Product Name	Application	Type
NBD-0040-YJ-VHH	Camelid Anti-FN1 Recombinant Single Domain Antibody (NBD-0040-YJ-VHH)	ELISA, In vivo imaging	Camelid VHH

CAT	Product Name	Application	Type
VS-0424-XY105	AbPlus™ Anti-FN1 Magnetic Beads (HFN7.1)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0724-YC853	AbPlus™ Anti-FN1 Magnetic Beads (VS-0724-YC853)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-1024-XY204	Mouse Anti-NHP FN1 Recombinant Antibody (clone C6F10)	WB, IHC, IF, IP	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS-0425-FY108	Human Anti-FN1 (clone L19) scFv-Fc Chimera	IHC, ELISA, IF	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY2593	Anti-FN1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY2594	Anti-Rat FN1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY2595	Anti-Mouse FN1 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC87	Recombinant Anti-FN1 ED-B (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone CGS-2 x Clone CGS-1)	ELISA	Tandem scFv

CAT	Product Name	Application	Type
VS-0625-YC121	Recombinant Anti-FN1 Eliminating Antibody, pH-Sensitive (VS-0625-YC121)	Antigen-Sweeping In Vivo.	Human IgG1 kappa

CAT	Product Name	Application	Type
VS-0825-YC138	SmartAb™ Recombinant Anti-FN1 pH-dependent Antibody (Clone L19)	ELISA, IF, IHC	Human IgG

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-Dabigatran Recombinant Antibody (TAB-H40) in HPLC

Humanized Anti-Dabigatran Recombinant Antibody (TAB-H40) (CAT# TAB-H40)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC

Figure 3 Anti-Human NCAM1 Therapeutic Antibody (TAB-188) in ELISA

Humanized Anti-NCAM1 Recombinant Antibody (clone Lorvotuzumab) (CAT# TAB-188)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 5 Anti-Human IL5RA Antibody (TAB-222) in DB

Anti-Human IL5RA Recombinant Antibody (TAB-222) (CAT# TAB-222)
Application: WB, FC, IP, ELISA, Neut, FuncS, IF

Figure 6 Anti-Human VEGF Recombinant Antibody (TAB-011) in ELISA

Anti-Human VEGF Recombinant Antibody (Bevacizumab) (CAT# TAB-011)
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC

Figure 1 Anti-Human ACVRL1 Recombinant Antibody (TAB-013CL) in SDS-PAGE

Anti-Human ACVRL1 Recombinant Antibody (TAB-013CL) (CAT# TAB-013CL)
Application:

Figure 6 Anti-LAM Recombinant Antibody (PABW-158, lot#CB2206LX02P6) in SEC-HPLC

Mouse Anti-LAM Recombinant Antibody (clone CS-35) (CAT# PABW-158)
Application: ELISA, IF, FC

Figure 2 Anti-Human TREM1 Recombinant Antibody (TAB-167CQ) in SDS-PAGE

Human Anti-TREM1 Recombinant Antibody (TAB-167CQ) (CAT# TAB-167CQ)
Application: ELISA, FC, WB, Block

Figure 2 CCP Recombinant Antibody (TAB-390CQ) in HPLC

Anti-Human CCP Recombinant Antibody (F12) (CAT# TAB-390CQ)
Application: ELISA, IHC

Figure 2 Recombinant Rat Anti-NGF Antibody (PABX-146) in SDS-PAGE

Recombinant Rat Anti-NGF Antibody (αD11) (CAT# PABX-146)
Application: Neut, FC, FuncS

Figure 2 Mouse Anti-ERBB2 Recombinant Antibody (NEUT-737CQ) in HPLC

Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) (CAT# NEUT-737CQ)
Application: Block, IP, IF, FC

Figure 2 Anti-Human MUC1 Recombinant Antibody (TAB-026ML) in HPLC

Anti-Human MUC1 Recombinant Antibody (TAB-026ML) (CAT# TAB-026ML)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 5 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in ELISA

Human Anti-FAP Recombinant Antibody (clone ESC14) (CAT# HPAB-0175-CN)
Application: ELISA, WB, FC, IHC, IP

Figure 2 Human Anti-CD70 Recombinant Antibody (HPAB-0102-LSX) in HPLC

Human Anti-CD70 Recombinant Antibody (HPAB-0102-LSX) (CAT# HPAB-0102-LSX)
Application: ELISA, FuncS

Figure 9 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in ELISA

Human Anti-Biotin Recombinant Antibody (HPAB-0109-YJ) (CAT# HPAB-0109-YJ)
Application: SPR

Figure 4 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in FACS

Human Anti-CD3 Recombinant Antibody scFv Fragment (HPAB-M0467-YC-S(P)) (CAT# HPAB-M0467-YC-S(P))
Application: FC

Figure 2 Human Anti-FRβ Recombinant Antibody (m909) (HPAB-0792-FY) in ELISA.

Human Anti-FRβ Recombinant Antibody (HPAB-0792-FY) (CAT# HPAB-0792-FY)
Application: ELISA

Figure 2 Recombinant Mouse Anti-Id (Idiotype) Antibody (HPAB-0644-WJ) in HPLC

Recombinant Mouse Anti-Id (Idiotype) Antibody (Ganglidiomab) (CAT# HPAB-0644-WJ)
Application: ELISA, WB

Figure 2 Recombinant Mouse Anti-Psa Fab Fragment (HPAB-3383LY-F(E)) in HPLC

Mouse Anti-Psa Recombinant Antibody; Fab Fragment (HPAB-3383LY-F(E)) (CAT# HPAB-3383LY-F(E))
Application: ELISA

Figure 4 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in ELISA

Human Anti-PEG Recombinant Antibody (clone E11) (CAT# FAMAB-1525CQ)
Application: WB, ELISA, FC

Figure 1 Mouse Anti-AIFM2 Antibody (ZG-0843F) in WB

Mouse Anti-AIFM2 Recombinant Antibody (clone HR231) (CAT# ZG-0843F)
Application: ELISA, WB, IP, IHC

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.