Mouse Anti-NHP FN1 Recombinant Antibody (clone C6F10)
CAT#: VS-1024-XY204
Designed for comprehensive analysis of FN1, this mouse monoclonal antibody demonstrates reactivity with avian, bovine, horse, human, non-human primate, and pig tissues. The antibody is suitable for a range of applications, including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). With its broad reactivity and versatile utility, it is an essential tool for studying FN1 expression and function across multiple species and experimental contexts.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
❮
❯
Specifications
- Immunogen
- Human plasma fibronectin
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- FN1
- Species Reactivity
- Avian, Bovine, Horse, Human, Non-human primate, Pig
- Clone
- C6F10
- Applications
- WB, IHC, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-2 µg/mL, IHC: 1-2 µg/mL, IF: 1-2 µg/mL, IP: 1-2 µg/100-500 µg of protein
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MOB-0025MZ | Recombinant Mouse Anti-Human Fibronectin Antibody (clone C715M) | ELISA | Mouse antibody |
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| VS3-CJ307 | Mouse Anti-FN1 Recombinant Antibody (VS3-CJ307) | WB, ICC, IF, IHC | |
| VS13-YC408 | Rabbit Anti-FN1 Recombinant Antibody (VS13-YC408) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-852CQ | Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1) | ELISA, WB, IP, Block | Mouse IgG1 |
| NEUT-853CQ | Mouse Anti-FN1 Recombinant Antibody (clone IST-9) | WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1326 | Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB) | IHC-Fr, IHC-P, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1110-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1110-FY-F(E)) | IHC | Human Fab |
| HPAB-1111-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1111-FY-F(E)) | IHC | Human Fab |
| HPAB-1112-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1112-FY-F(E)) | IHC | Human Fab |
| HPAB-1113-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1113-FY-F(E)) | IHC | Human Fab |
| HPAB-1114-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1114-FY-F(E)) | IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1110-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1110-FY-S(P)) | IHC | Human scFv |
| HPAB-1111-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1111-FY-S(P)) | IHC | Human scFv |
| HPAB-1112-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1112-FY-S(P)) | IHC | Human scFv |
| HPAB-S0037-YC-F(E) | Human Anti-FN1 Recombinant Antibody (clone CGS-2); Fab Fragment | ELISA, IB, IHC | Human Fab |
| HPAB-S0038-YC-F(E) | Human Anti-FN1 Recombinant Antibody (clone L19); Fab Fragment | ELISA, IF, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0040-YJ-VHH | Camelid Anti-FN1 Recombinant Single Domain Antibody (NBD-0040-YJ-VHH) | ELISA, In vivo imaging | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY105 | AbPlus™ Anti-FN1 Magnetic Beads (HFN7.1) | IP, Protein Purification | |
| VS-0724-YC853 | AbPlus™ Anti-FN1 Magnetic Beads (VS-0724-YC853) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY108 | Human Anti-FN1 (clone L19) scFv-Fc Chimera | IHC, ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2593 | Anti-FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2594 | Anti-Rat FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2595 | Anti-Mouse FN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC87 | Recombinant Anti-FN1 ED-B (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone CGS-2 x Clone CGS-1) | ELISA | Tandem scFv |
| CAT | Product Name | Application | Type |
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| VS-0625-YC121 | Recombinant Anti-FN1 Eliminating Antibody, pH-Sensitive (VS-0625-YC121) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
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