Recombinant Human Anti-HIV-1 Antibody (Fab4025) (CAT#: PABX-094)

Anti-HIV-1 antibody is a Human antibody of IgG class that binds to an HIV-1. It developed From individual infected with V3 HIV-1.


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Figure 1 Relative affinities of mAbs binding to the mimotope as measured by 50% of maximal binding (‘‘halfmax’’) to plates coated with the biotinylated mimotope at a concentration of 1.0 mg/ml. Lower half-max values reflect higher relative affinities.

Figure 1 Relative affinities of mAbs binding to the mimotope as measured by 50% of maximal binding (‘‘halfmax’’) to plates coated with the biotinylated mimotope at a concentration of 1.0 mg/ml. Lower half-max values reflect higher relative affinities.

Gorny, M. K., Sampson, J., Li, H., Jiang, X., Totrov, M., Wang, X. H., ... & Cardozo, T. (2011). Human anti-V3 HIV-1 monoclonal antibodies encoded by the VH5-51/VL lambda genes define a conserved antigenic structure. PloS one, 6(12), e27780.

Figure 2 Structural similarity of the antigen-binding sites of VH5-51 encoded anti-V3 mAbs.

Figure 2 Structural similarity of the antigen-binding sites of VH5-51 encoded anti-V3 mAbs.

(A) A structural superimposition of the Ca atoms of the Fab variable domains of complexes 1006/MN (green), 2219/MN (cyan), 2557/NY5 (magenta), 2558/MN (yellow), and 4025/ConA (salmon). Note the domains of CDR H1, H2, L1 and L2 superimposed well, while that of CDR L3 and H3 is more structurally divergent. (B) Same superimposition as that in panel (A) but with the key contact residues of the mAbs labeled (all contact residues are shown in Tables 4 and 5). The position of the V3 is illustrated as a dashed line. (C) and (D) Structural details of the antigen combining sites of the light (C) and heavy chains (D) in the five complexes.

Gorny, M. K., Sampson, J., Li, H., Jiang, X., Totrov, M., Wang, X. H., ... & Cardozo, T. (2011). Human anti-V3 HIV-1 monoclonal antibodies encoded by the VH5-51/VL lambda genes define a conserved antigenic structure. PloS one, 6(12), e27780.

Figure 3 Schematic of antigenic profile analysis on intact liposomes compared to soluble trimers. bnAb (PGT121, PGT145, PGT151, VRC01) and non-nAb (B6, 39 F, 4025) binding profiles of non-covalent and covalent MD39 liposomes. 
mouse serum (blue) at 37 °C (n = 3).

Figure 3 Schematic of antigenic profile analysis on intact liposomes compared to soluble trimers. bnAb (PGT121, PGT145, PGT151, VRC01) and non-nAb (B6, 39 F, 4025) binding profiles of non-covalent and covalent MD39 liposomes. mouse serum (blue) at 37 °C (n = 3).

Tokatlian, T., Kulp, D. W., Mutafyan, A. A., Jones, C. A., Menis, S., Georgeson, E., ... & Yun, D. S. (2018). Enhancing humoral responses against HIV envelope trimers via nanoparticle delivery with stabilized synthetic liposomes. Scientific reports, 8(1), 16527.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native HIV-1
  • Species Reactivity
  • Human immunodeficiency virus 1
  • Clone
  • Fab4025
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS(assay-depended)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-1889-sdAb Recombinant Anti-HIV-1 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH
PNBL-038 Recombinant Anti-HIV-1 C186 gp120 VHH Single Domain Antibody (A12) WB, FuncS Llama VHH

Humanized Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G037E Anti-HIV-1 immunotoxin 3B3 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G037D Anti-HIV-1 immunotoxin 3B3 (scFv)-DT Cytotoxicity assay, Function study

Chimeric Antibody

CAT Product Name Application Type
PABX-093-S (P) Recombinant Mouse Anti-HIV-1 Antibody scFv Fragment (Fab28) WB, ELISA, FuncS scFv

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4221 Rabbit Anti-HIV-1 Recombinant Antibody (clone SI300DS) ELISA, ICC, IF, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-068 Afuco™ Anti-HIV-1 ADCC Recombinant Antibody (Suvizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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