Recombinant Human Anti-HIV-1 Antibody Fab Fragment (Fab4025) (CAT#: PABX-094-F (E))

Figure 1 Relative affinities of mAbs binding to the mimotope as measured by 50% of maximal binding (‘‘halfmax’’) to plates coated with the biotinylated mimotope at a concentration of 1.0 mg/ml. Lower half-max values reflect higher relative affinities.

Gorny, M. K., Sampson, J., Li, H., Jiang, X., Totrov, M., Wang, X. H., ... & Cardozo, T. (2011). Human anti-V3 HIV-1 monoclonal antibodies encoded by the VH5-51/VL lambda genes define a conserved antigenic structure. PloS one, 6(12), e27780.

Figure 2 Structural similarity of the antigen-binding sites of VH5-51 encoded anti-V3 mAbs.

(A) A structural superimposition of the Ca atoms of the Fab variable domains of complexes 1006/MN (green), 2219/MN (cyan), 2557/NY5 (magenta), 2558/MN (yellow), and 4025/ConA (salmon). Note the domains of CDR H1, H2, L1 and L2 superimposed well, while that of CDR L3 and H3 is more structurally divergent. (B) Same superimposition as that in panel (A) but with the key contact residues of the mAbs labeled (all contact residues are shown in Tables 4 and 5). The position of the V3 is illustrated as a dashed line. (C) and (D) Structural details of the antigen combining sites of the light (C) and heavy chains (D) in the five complexes.

Gorny, M. K., Sampson, J., Li, H., Jiang, X., Totrov, M., Wang, X. H., ... & Cardozo, T. (2011). Human anti-V3 HIV-1 monoclonal antibodies encoded by the VH5-51/VL lambda genes define a conserved antigenic structure. PloS one, 6(12), e27780.

Figure 3 Schematic of antigenic profile analysis on intact liposomes compared to soluble trimers. bnAb (PGT121, PGT145, PGT151, VRC01) and non-nAb (B6, 39 F, 4025) binding profiles of non-covalent and covalent MD39 liposomes. mouse serum (blue) at 37 °C (n = 3).

Tokatlian, T., Kulp, D. W., Mutafyan, A. A., Jones, C. A., Menis, S., Georgeson, E., ... & Yun, D. S. (2018). Enhancing humoral responses against HIV envelope trimers via nanoparticle delivery with stabilized synthetic liposomes. Scientific reports, 8(1), 16527.