Recombinant Mouse Anti-HIV-1 Antibody scFv Fragment (A10F9) (CAT#: PABX-089-S (P))

Figure 1 SDS-PAGE and western blotting of the assembled CA N21C/A22C-V5.

Lane M, molecular weight marker; lane 1, protein before assembly; lane 2-5, protein after assembly. The primary antibody is a CA specific mAb A10F11.

Zhang, Z., He, M., Bai, S., Zhang, F., Jiang, J., Zheng, Q., ... & Xia, N. (2018). T= 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display. Viruses, 10(12), 667.

Figure 2 Antigenicity of CA N21C/A22C-V3 was determined with the A10F11 mAb using indirect ELISA.

Data were analyzed by GraphPad Prism software. The 50% maximal effective concentration (EC52) was calculated by a four-parameter logistic fit, and is indicated in the square brackets.

Zhang, Z., He, M., Bai, S., Zhang, F., Jiang, J., Zheng, Q., ... & Xia, N. (2018). T= 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display. Viruses, 10(12), 667.

Figure 3 Ribbon representation of a detailed view of p24 CTD recognition by the A10F11 Fab fragment.

The residues involved in protein-protein interactions are indicated and shown in stick mode.

Gu, Y., Cao, F., Wang, L., Hou, W., Zhang, J., Hew, C. L., ... & Xia, N. (2013). Structure of a novel shoulder-to-shoulder p24 dimer in complex with the broad-spectrum antibody A10F9 and its implication in capsid assembly. PloS one, 8(4), e61316.