Recombinant human antibody to Human CXCR4
Figure 1 SIA induction by Ulocuplumab (BMS-936564) is CXCR4 specific as observed in Ramos and primary CLL cells but not in K562.
Ramos and K562 cell lines were treated with different concentrations of Ulocuplumab (BMS-936564) and AMD3100 for 48 hrs followed by running the samples on flow cytometer to study the % SIA. Treatment with AMD3100 in Ramos/ K562/CLL did not induce significant % SIA as compared to Ulocuplumab (BMS-936564). In contrast, Ulocuplumab (BMS-936564) induced significant % SIA in Ramos and CLL cells (CXCR4+), but not in K562 (CXCR4-) as evaluated by flow cytometry).
Kashyap, M. K. , Kumar, D. , Jones, H. , Amayachanaga, C. I. , Choi, M. Y. , & Melocardenas, J. , et al. (2016). Ulocuplumab (bms-936564 / mdx1338): a fully human anti-cxcr4 antibody induces cell death in chronic lymphocytic leukemia mediated through a reactive oxygen species-dependent pathway. Oncotarget, 7(3), 2809-2822.
Figure 2 SIA induction by Ulocuplumab (BMS-936564) is CXCR4 specific as observed in Ramos and primary CLL cells but not in K562.
The CLL cells cultured either alone or with stromal cell support were treated with Ulocuplumab (BMS-936564) or AMD3100 for 48 hrs at 37 o C followed by flow cytometer for measurement of % SIA. AMD3100 does not induce significant % SIA in CLL as compared to Ulocuplumab (BMS-936564). Ulocuplumab (BMS-936564) induced significant amount of % SIA in CLL cells alone or CLL co-cultured with stromal cell support.
Kashyap, M. K. , Kumar, D. , Jones, H. , Amayachanaga, C. I. , Choi, M. Y. , & Melocardenas, J. , et al. (2016). Ulocuplumab (bms-936564 / mdx1338): a fully human anti-cxcr4 antibody induces cell death in chronic lymphocytic leukemia mediated through a reactive oxygen species-dependent pathway. Oncotarget, 7(3), 2809-2822.
Figure 3 Ulocuplumab Inhibits the EMTPhenotype Acquisition in MM Cells BothIn Vitro and In Vivo.
MM.1S cells were cultured in presenceor absence of ulocuplumab (0.025–1 mM; 6 hr).MM.1S cells were then harvested, and cell lysateswere subjected to western blot using anti-p-b-catenin,-b-catenin, -p-GSK3-b, -tubulin, -Snail,-Twist, -Slug, and -E-cadherin antibodies. Adjustedband intensity for each protein normalized to therelative loading control is provided within FiguresS5A and S5B and expressed as fold of control(control corresponds to untreated cells).
Roccaro, A. , Mishima, Y. , Sacco, A. , Moschetta, M. , Tai, Y. T. , & Shi, J. , et al. (2015). Cxcr4 regulates extra-medullary myeloma through epithelial-mesenchymal-transition-like transcriptional activation. Cell Reports, 12(4), 622-635.
Figure 4 Ulocuplumab Inhibits the EMTPhenotype Acquisition in MM Cells BothIn Vitro and In Vivo.
Bone chips from donor mice wereloaded with CXCR4+ MM.1S and implanted sub-cutaneously into SCID/Bg-recipient mice. Micewere treated with either control Ab or ulocuplumab(n = 5/group; 10 mg/kg; i.p.; 43to 53/week).Ulocuplumab led to inhibited MM cell growthwithin the implanted bone (implanted bone isshown in C), together with inhibited ability of MMcells to metastatize from bone to bone (host boneis shown in D).
Roccaro, A. , Mishima, Y. , Sacco, A. , Moschetta, M. , Tai, Y. T. , & Shi, J. , et al. (2015). Cxcr4 regulates extra-medullary myeloma through epithelial-mesenchymal-transition-like transcriptional activation. Cell Reports, 12(4), 622-635.
Figure 5 Ulocuplumab Inhibits the EMTPhenotype Acquisition in MM Cells BothIn Vitro and In Vivo.
The ability of ulocuplumab to modulate EMTwas demonstrated ex vivo: decreased mRNAlevels of Twist, Snail, and Slug, together withincreased mRNA levels of E-cadherin, wereobserved in BM cells obtained from the host fe-murs of ulocuplumab-treated mice. No MM cell-injected mice were used as control. p indicates pvalue. Average of experiments performed in tripli-cate is shown. Error bars indicate SD.
Roccaro, A. , Mishima, Y. , Sacco, A. , Moschetta, M. , Tai, Y. T. , & Shi, J. , et al. (2015). Cxcr4 regulates extra-medullary myeloma through epithelial-mesenchymal-transition-like transcriptional activation. Cell Reports, 12(4), 622-635.
Figure 6 Ulocuplumab Exerts Anti-MMActivity, Either as Single Agent or in Combi-natory Regimens In Vivo, and InducesToxicity on Primary MM Cells.
SCID/Bg mice were injected with 5 3106MM.1S-GFP+/Luc+cells i.v. and treated with ulo-cuplumab (10 mg/kg; 33to 43/week; i.p.) or iso-type control antibody (30 mg/kg; 33to 43/week;i.p.). Ulocuplumab led to inhibited MM cell homingto the BM, as shown by using intravital confocalmicroscopy at the 3rdweek. (GFP+MM cells, greencolor; Evans-Blue-positive blood vessels, redcolor). High-resolution images with cellular detailwere obtained through the intact mouse skull atdepths of up to 250 mm from the surface of the skullusing a 1030.45NA Plan-Apo objective (CarlZeiss) and assembled together to generate a finalimage that depicts the whole bone marrow.
Roccaro, A. , Mishima, Y. , Sacco, A. , Moschetta, M. , Tai, Y. T. , & Shi, J. , et al. (2015). Cxcr4 regulates extra-medullary myeloma through epithelial-mesenchymal-transition-like transcriptional activation. Cell Reports, 12(4), 622-635.
Figure 7 Ulocuplumab Exerts Anti-MMActivity, Either as Single Agent or in Combi-natory Regimens In Vivo, and InducesToxicity on Primary MM Cells.
BM-derived primary CD138+ cells werecultured in presence or absence of ulocuplumab(50–400 nM; 48 hr). Cell toxicity was performed byusing MTT. Ulocuplumab exerted anti-MM activityagainst primary MM cells. Error bars indicate SD.
Roccaro, A. , Mishima, Y. , Sacco, A. , Moschetta, M. , Tai, Y. T. , & Shi, J. , et al. (2015). Cxcr4 regulates extra-medullary myeloma through epithelial-mesenchymal-transition-like transcriptional activation. Cell Reports, 12(4), 622-635.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Afuco™ Anti-CXCR4 ADCC Recombinant Antibody (Ulocuplumab), ADCC EnhancedThis product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant human antibody to Human CXCR4.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0011CL-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (414H5) | FC, IHC, Block, Inhib, FuncS | |
TAB-0012CL-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (515H7) | FC, IHC, Block, Inhib, FuncS | |
TAB-0314CL-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (TAB-0314CL-F(E)) | FC | Mouse Fab |
TAB-143MZ-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-143MZ-S(P)) | cAMP Assay | Mouse scFv |
TAB-144MZ-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-144MZ-S(P)) | cAMP Assay | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-146MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-146MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
TAB-147MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-147MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
TAB-146MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-146MZ-S(P)) | cAMP Assay | Humanized scFv |
TAB-147MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-147MZ-S(P)) | cAMP Assay | Humanized scFv |
TAB-148MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-148MZ-S(P)) | cAMP Assay | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-150MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (CF172) | FC | Human antibody |
TAB-151MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5C9) | WB, ELISA | Human antibody |
TAB-152MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5E1) | WB, ELISA | Human antibody |
TAB-153MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (7C8) | WB, ELISA | Human antibody |
TAB-154MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (6C7) | WB, ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0152MZ | Chicken Anti-CXCR4 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-684CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5) | FC, CyTOF®, Neut | Mouse IgG2a |
NEUT-685CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5R) | FC, Neut | Mouse IgG2a |
NEUT-687CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL046) | FC, IHC, Neut | Mouse IgG2 |
NEUT-688CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL470) | FC, CyTOF®, Neut | Mouse IgG2b |
NEUT-689CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL783) | Neut | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-686CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 44716.111) | FC, ICC, IF, IHC-P, Inhib | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0889 | Hi-Affi™ Rabbit Anti-CXCR4 Recombinant Antibody (clone DS889AB) | WB, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0044-YC | Human Anti-CXCR4 Recombinant Antibody (clone hz515H7VH1D76NVL2) | Block, Cyt, FC | Humanized IgG1, κ |
HPAB-0045-YC | Human Anti-CXCR4 Recombinant Antibody (HPAB-0045-YC) | FC, Cyt | Humanized IgG |
FN-163CQ | Rat Anti-Cxcr4 Recombinant Antibody (clone CBL-1324) | FC, FuncS | Rat IgG2b, κ |
HPAB-0685-WJ | Human Anti-CXCR4 Recombinant Antibody (HPAB-0685-WJ) | FC | Human IgG |
HPAB-0686-WJ | Human Anti-CXCR4 Recombinant Antibody (HPAB-0686-WJ) | FC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H72 | Afuco™ Anti-CXCR4 ADCC Recombinant Antibody (Ulocuplumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0329-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0329-WJ-F(E)) | ELISA, FC, IHC | Mouse Fab |
HPAB-0334-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 4C1); Fab Fragment | ELISA, WB | Mouse Fab |
HPAB-0335-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 6C7); Fab Fragment | ELISA, WB | Mouse Fab |
HPAB-0336-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 2A4); Fab Fragment | ELISA, WB | Mouse Fab |
HPAB-0337-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 5C9); Fab Fragment | ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1504-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone C-9P21); scFv Fragment | ELISA, Inhib | Human scFv |
HPAB-1505-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone B-1M2); scFv Fragment | ELISA, Inhib | Human scFv |
HPAB-1506-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone C-1I24); scFv Fragment | ELISA, Inhib | Human scFv |
HPAB-AP621-YC-S(P) | Mouse Anti-CXCR4 Recombinant Antibody (clone c515H7); scFv Fragment | ELISA, FC (10 μg/ml), Inhib, FuncS | Mouse scFv |
HPAB-1843-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone Ab125); scFv Fragment | Inhib | Human scFv |
There are currently no Customer reviews or questions for TAB-H72. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.