Human Anti-Human CXCR4 Recombinant Antibody (TAB-H72) (CAT#: TAB-H72)
Recombinant human antibody to Human CXCR4
Specific Inquiry
Figure 1 SIA induction by Ulocuplumab (BMS-936564) is CXCR4 specific as observed in Ramos and primary CLL cells but not in K562.
Ramos and K562 cell lines were treated with different concentrations of Ulocuplumab (BMS-936564) and AMD3100 for 48 hrs followed by running the samples on flow cytometer to study the % SIA. Treatment with AMD3100 in Ramos/ K562/CLL did not induce significant % SIA as compared to Ulocuplumab (BMS-936564). In contrast, Ulocuplumab (BMS-936564) induced significant % SIA in Ramos and CLL cells (CXCR4+), but not in K562 (CXCR4-) as evaluated by flow cytometry).
Kashyap, M. K. , Kumar, D. , Jones, H. , Amayachanaga, C. I. , Choi, M. Y. , & Melocardenas, J. , et al. (2016). Ulocuplumab (bms-936564 / mdx1338): a fully human anti-cxcr4 antibody induces cell death in chronic lymphocytic leukemia mediated through a reactive oxygen species-dependent pathway. Oncotarget, 7(3), 2809-2822.
Figure 2 SIA induction by Ulocuplumab (BMS-936564) is CXCR4 specific as observed in Ramos and primary CLL cells but not in K562.
The CLL cells cultured either alone or with stromal cell support were treated with Ulocuplumab (BMS-936564) or AMD3100 for 48 hrs at 37 o C followed by flow cytometer for measurement of % SIA. AMD3100 does not induce significant % SIA in CLL as compared to Ulocuplumab (BMS-936564). Ulocuplumab (BMS-936564) induced significant amount of % SIA in CLL cells alone or CLL co-cultured with stromal cell support.
Kashyap, M. K. , Kumar, D. , Jones, H. , Amayachanaga, C. I. , Choi, M. Y. , & Melocardenas, J. , et al. (2016). Ulocuplumab (bms-936564 / mdx1338): a fully human anti-cxcr4 antibody induces cell death in chronic lymphocytic leukemia mediated through a reactive oxygen species-dependent pathway. Oncotarget, 7(3), 2809-2822.
Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG4 - kappa
- Specificity
- CXCR4(chemokine (C-X-C motif) receptor 4, fusin, stromal cell-derived factor 1 receptor, SDF-1 receptor, CXCL12 receptor, CD184) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- IP, IF, FuncS, FC, Neut, ELISA, ICC
- MW
- 146.2 kDa
- Related Disease
- Acute myeloid leukemia (AML)
Product Property
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
Applications
- Application Notes
- The CXCR4 antibody has been reported in applications of FC, Cyt, WB, IHC, RT-PCR, IF, MTT.
WB: Cells were harvested and lysed using lysis buffer supplemented with 5 mM NaF, 2 mM Na3VO4, 1 mM PMSF (polymethylsulfonylfluoride), 5 mg/ml leupeptin, and 5 mg/ml aprotinin.
IHC: Autopsy specimens of synchronous secondary extramedullary MM sites wereanalyzed for the expression of CD138 and CXCR4. Murine BM tissues (femurs)were analyzed for the expression of human CD138 and stained with hema-toxylin-eosin.
Target
- Alternative Names
- FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; WHIMS1; D2S201E
- Gene ID
- 7852
- UniProt ID
- P61073
Related Resources
Please refer to Ulocuplumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ulocuplumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0011CL-S(P) | Anti-Human CXCR4 Recombinant Antibody scFv Fragment (414H5) | FC, IHC, Block, Inhib, FuncS | |
| TAB-0012CL-S(P) | Anti-Human CXCR4 Recombinant Antibody scFv Fragment (515H7) | FC, IHC, Block, Inhib, FuncS | |
| TAB-0314CL-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-0314CL-S(P)) | FC | Mouse scFv |
| TAB-0314CL-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (TAB-0314CL-F(E)) | FC | Mouse Fab |
| TAB-143MZ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (TAB-143MZ-F(E)) | cAMP Assay | Mouse Fab |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H72, RRID: AB_3112044)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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