Does this antibody recognize the IL-23 p19 subunit?

Yes, this Recombinant Mouse Monoclonal Antibody (clone IMU3847) was raised against Human recombinant p40 and p19 with an elastin linker, and it specifically recognizes the Human IL23A (p19) subunit.

What applications can be performed with this antibody?

This antibody is validated for Immunohistochemistry on paraffin-embedded sections (IHC-P), Immunoprecipitation (IP), and Western Blot (WB). It is Protein G purified and supplied unconjugated.

Recombinant Mouse anti-Human IL23A Monoclonal antibody (IMU3847)

CAT#: MOB-0967CT

Recombinant Mouse monoclonal antibody to Human IL23A, expressed in Chinese Hamster Ovary cells(CHO).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells in and outside germinal center.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/60_H7_1_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_9_3.jpg

Normal Tissue Figure 5 Testis

Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_5_6.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1554/4856_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110944-IL23A

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Specifications

Immunogen
This immunogen is full-length recombinant human IL-23 p19 protein.

Host Species
Mouse

Specificity
Human recombinant p40 and p19 with elastin linker.

Species Reactivity
Human

Clone
IMU3847

Applications
Used for immunoassay techniques such as: Immunohistochemistry paraffin embedded sections; Immunoprecipitation; Western Blot

Conjugate
Unconjugated

Product Property

Purity
Protein G purified

Format
Liquid

Concentration
0.5 mg/ml

Buffer
pH: 7.20Preservative:0.09% Sodium azideConstituent:PBS

Storage
Store at +4°C.

Target

Alternative Names
IL23A; interleukin 23, alpha subunit p19; P19; SGRF; IL-23; IL-23A; IL23P19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor;

Gene ID
51561

UniProt ID
Q9NPF7

Protein Refseq
NP_057668

Function
cytokine activity; contributes_to interleukin-23 receptor binding;

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Breast cancer biomarkers at key points during disease progression

Specific IL23A Detection

We used the clone IMU3847 to detect the p19 subunit of IL-23 (IL23A) via Western Blot. It specifically recognized the recombinant human protein. This Recombinant Mouse Monoclonal Antibody is Protein G purified, ensuring low background in our assays.

Good For IHC-P

We also employed this antibody for Immunohistochemistry on paraffin-embedded sections. It provided specific staining of IL23A-positive cells. It is an IgG1 antibody produced in CHO cells, offering consistent quality. We store it at 4°C for immediate use during our experiments.

Q&As

Does this antibody recognize the IL-23 p19 subunit?

A: Yes, this Recombinant Mouse Monoclonal Antibody (clone IMU3847) was raised against Human recombinant p40 and p19 with an elastin linker, and it specifically recognizes the Human IL23A (p19) subunit.
What applications can be performed with this antibody?

A: This antibody is validated for Immunohistochemistry on paraffin-embedded sections (IHC-P), Immunoprecipitation (IP), and Western Blot (WB). It is Protein G purified and supplied unconjugated.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-0967CT.pdf

MSDS

MSDS - MOB-0967CT.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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