Recombinant Human Anti-IFN-gamma Antibody (HuZAF) (CAT#: PABX-113)

Anti-IFN-gamma antibody is a Humanized antibody of IgG class that binds to an IFN-gamma.

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Figure 1 Competitive binding analysis of AF2 and HuZAF.

A, Graph represents the relative ability of AF2 and HuZAF to compete with bAF2 for binding to human IFN-g in an ELISA format. Increasing concentrations of either AF2 (F) or HuZAF (■) were added to wells coated with human IFN-g, after which bAF2 was added. Error bars indicate the SE of triplicate samples. B, Neutralization of IFN-g-mediated induction of MHC class II
molecule expression on Hs294T cells. Graph represents the median fluorescent intensity of HLA-DR expression on Hs294T cells incubated with IFN-g and increasing concentrations of either AF2 (F) or HuZAF (■).
Median fluorescent intensity of Hs294T cells incubated in the absence of IFN-g was 3.74; median fluorescent intensity in the presence of IFN-g, but in the absence of Ab was 9.65.

Landolfi, N. F., Thakur, A. B., Fu, H., Vásquez, M., Queen, C., & Tsurushita, N. (2001). The integrity of the ball-and-socket joint between V and C domains is essential for complete activity of a humanized antibody. The Journal of Immunology, 166(3), 1748-1754.

Figure 2 Involvement of AF2 VH residues 11 and 38 in neutralization of IFN-g activity.

Graph represents the median fluorescent intensity of HLA-DR expression on Hs294T cells incubated with IFN-g and increasing concentrations of either EuAF2 (F), or EuAF2 with the following substitutions: R38K (l), V11L (triangles), or V11L, R38K (■).

Landolfi, N. F., Thakur, A. B., Fu, H., Vásquez, M., Queen, C., & Tsurushita, N. (2001). The integrity of the ball-and-socket joint between V and C domains is essential for complete activity of a humanized antibody. The Journal of Immunology, 166(3), 1748-1754.

Figure 3 Stereo illustration of the three-dimensional structure of the HuZAF Fab, prepared with the program MOLSCRIPT (Kraulis, 1991).

Secondary elements are superimposed on backbone tracings, which are colored yellow in the L chain and steel blue in the H chain. -Strands are represented by directional arrows: cyan for the four-stranded -pleated sheets and magenta for the ®ve-stranded (VL) and three-stranded layers (CL) of the L chain and red and white for the corresponding -pleated sheets in the H chain. Helices are identi®ed by red and yellow spirals. CDRs are marked 1, 2 and 3.

Bourne, P. C., Terzyan, S. S., Cloud, G., Landolfi, N. F., Vásquez, M., & Edmundson, A. B. (2004). Three-dimensional structures of a humanized anti-IFN-γ Fab (HuZAF) in two crystal forms. Acta Crystallographica Section D: Biological Crystallography, 60(10), 1761-1769.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human IFN-gamma
  • Species Reactivity
  • Human
  • Clone
  • HuZAF
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (assay-depended)

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • IFNG; interferon; gamma; IFG; IFI; interferon gamma; IFN-gamma; immune interferon; NI-0501

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "IFNG"

See other products for "Clone HuZAF"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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