Recombinant Humanized Antibody (AF-2) is capable of binding to IFNG, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IFNG mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti- IFNG mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The AF2 antibody prevented the up regulation to a greater extent than any other member of the panel.
Figure 1 Effect of AF-2, a neutralizing Ab to human IFN-γ, on PMA-induced E-selectin expression on HUVEC, pretreated with IFN-γ.
HUVEC were cultured with 200 ng/ml IFN-γ ▪ or medium alone □ for 24 h in the presence or absence of the indicated Abs at 10 μg/ml. PMA, 1 ng/ml, was then added for an additional 4 h, and cells were analyzed for expression of E-selectin by a cell-based ELISA, as described in Materials and Methods. The relative amount of E-selectin measured is indicated by the OD at 450 nm. The mean ± SD from triplicate samples in a single, representative experiment is shown. **Indicates p < 0.005; *indicates p < 0.05; NS indicates not significant, comparing with and without IFN-γ.
Melrose, J. , Tsurushita, N. , Liu, G. , & Berg, E. L. . (1998). Ifn-γ inhibits activation-induced expression of e- and p-selectin on endothelial cells. Journal of Immunology, 161(5), 2457-64.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABL-090 | Recombinant Anti-human IFNG VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
TAB-140CT | Anti-Human IFN-γ Therapeutic Single Domain Antibody (BIV 3E) | ELISA | Single domain antibody |
TAB-141CT | Anti-Human IFN-γ Therapeutic Single Domain Antibody (TRI 3E) | ELISA | Single domain antibody |
TAB-142CT | Anti-Human IFN-γ Therapeutic Single Domain Antibody (TETRA 3E) | ELISA | Single domain antibody |
TAB-143CT | Anti-Human IFN-γ Therapeutic Single Domain Antibody (BIV#m3F) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-041 | Anti-Human IFNG Recombinant Antibody (Fontolizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | IgG1 |
PABX-113 | Recombinant Human Anti-IFN-gamma Antibody (HuZAF) | WB, ELISA, FuncS | IgG |
PABX-113-F (E) | Recombinant Human Anti-IFN-gamma Antibody Fab Fragment (HuZAF) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0289MC | Recombinant Anti-Canine IFN-gamma Antibody. | WB, ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-112 | Recombinant Human Anti-IFN Antibody (ScFv01) | Neut, FuncS | IgG |
PABX-112-S (P) | Recombinant Human Anti-IFN Antibody scFv Fragment (ScFv01) | Neut, FuncS | scFv |
PABX-113-S (P) | Recombinant Human Anti-IFN-gamma Antibody scFv Fragment (HuZAF) | WB, ELISA, FuncS | scFv |
PABX-112-F (E) | Recombinant Human Anti-IFN Antibody Fab Fragment (ScFv01) | Neut, FuncS | Fab |
TAB-423CQ | Anti-Human IFNG Recombinant Antibody (Emapalumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, λ1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-998CQ | Mouse Anti-Ifng Recombinant Antibody (clone CBL210), Biotin | ELISA, Neut | Mouse IgG1 |
NEUT-999CQ | Mouse Anti-IFNG Recombinant Antibody (clone CBL232) | ELISA, WB, Neut | Mouse IgG1 |
NEUT-1000CQ | Mouse Anti-IFNG Recombinant Antibody (clone CBL241) | Neut, FC, ELISA | Mouse IgG1 |
NEUT-1001CQ | Mouse Anti-IFNG Recombinant Antibody (clone CBL246), Biotin | ELISA, FC, ICC, IP, Neut | Mouse IgG1, κ |
NEUT-1003CQ | Mouse Anti-IFNG Recombinant Antibody (clone CBL258), FITC | FC, ICC, Neut | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1002CQ | Mouse Anti-IFNG Recombinant Antibody (clone CBL253) | Inhib, IHC, IP | Mouse IgG1 |
NEUT-1008CQ | Mouse Anti-IFNG Recombinant Antibody (clone B133.5) | Block | Mouse IgG1 |
NEUT-1013CQ | Mouse Anti-IFNG Recombinant Antibody (clone B-B1) | Block, FC | Mouse IgG1 |
NEUT-1014CQ | Mouse Anti-IFNG Recombinant Antibody (clone D9D10) | Block, FC, ELISA, WB | Mouse IgG1 |
NEUT-1033CQ | Mouse Anti-IFNG Recombinant Antibody (clone SN-6) | ELISA, Block | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1742 | Rabbit Anti-IFNG Recombinant Antibody (clone DS1742AB) | WB | Rabbit IgG |
MOR-4228 | Rabbit Anti-IFNG Recombinant Antibody (clone SI307DS) | FC | Rabbit IgG |
MOR-4600 | Rabbit Anti-IFNG Recombinant Antibody (clone TH113DS) | WB | Rabbit IgG |
MOR-4601 | Rabbit Anti-IFNG Recombinant Antibody (clone TH114DS) | WB | Rabbit IgG |
MOR-4602 | Rabbit Anti-Ifng Recombinant Antibody (clone TH115DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0882LC | Mouse Anti-IFNG Recombinant Antibody (clone MIF3009) | ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0162-CN | Human Anti-IFNG Recombinant Antibody (clone 41ms4G) | ELISA | Human IgG |
FAMAB-0163-CN | Human Anti-IFNG Recombinant Antibody (clone 57-5) | ELISA | Human IgG |
FAMAB-0164-CN | Human Anti-IFNG Recombinant Antibody (clone 36-4) | ELISA | Human IgG |
FAMAB-0165-CN | Human Anti-IFNG Recombinant Antibody (clone 44ms4) | ELISA | Human IgG |
FAMAB-0166-CN | Human Anti-IFNG Recombinant Antibody (clone 13ms5F) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0181-CN-S(P) | Human Anti-IFNG Recombinant Antibody (clone 5MD2); scFv Fragment | ELISA | Human scFv |
FAMAB-0182-CN-S(P) | Human Anti-IFNG Recombinant Antibody (clone 4NB12); scFv Fragment | ELISA | Human scFv |
FAMAB-0183-CN-S(P) | Human Anti-IFNG Recombinant Antibody (clone 14); scFv Fragment | ELISA | Human scFv |
HPAB-S0068-YC-S(P) | Human Anti-IFNG Recombinant Antibody (clone Haf25); scFv Fragment | ELISA, Neut | Humanized scFv |
HPAB-S0069-YC-S(P) | Human Anti-IFNG Recombinant Antibody (clone HuXAF); scFv Fragment | ELISA, Neut | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0162-CN-F(E) | Human Anti-IFNG Recombinant Antibody (clone 41ms4G); Fab Fragment | ELISA | Human Fab |
FAMAB-0163-CN-F(E) | Human Anti-IFNG Recombinant Antibody (clone 57-5); Fab Fragment | ELISA | Human Fab |
FAMAB-0164-CN-F(E) | Human Anti-IFNG Recombinant Antibody (clone 36-4); Fab Fragment | ELISA | Human Fab |
FAMAB-0165-CN-F(E) | Human Anti-IFNG Recombinant Antibody (clone 44ms4); Fab Fragment | ELISA | Human Fab |
FAMAB-0166-CN-F(E) | Human Anti-IFNG Recombinant Antibody (clone 13ms5F); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-041 | Afuco™ Anti-IFNG ADCC Recombinant Antibody (Fontolizumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
AFC-TAB-423CQ | Afuco™ Anti-IFNG ADCC Recombinant Antibody (Emapalumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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