Recombinant Mouse Anti-Mouse J1 Antibody (M2139)
CAT#: PABL-649
Recombinant Mouse Antibody (M2139) is capable of binding to Mouse J1 , expressed in Chinese Hamster Ovary cells (CHO). This mAb specifically recognizes the J1 epitope and is the most arthritogenic anti-CII mAb in the mouse, eliciting disease upon single transfer.
Published Data
Gene Expression
Figure 1 Western blot analysis antibody M2139 binding to recombinant IdeS.
Recombinant IdeS was incubated with purified monoclonal antibody (mAb) M287 (IgG2a), CIIC1 (IgG2a), M2139 (IgG2b), M284 (IgG1), or B10 (IgG3), separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis under reducing conditions on Precise Protein Gradient Gels, and stained with Coomassie blue.
Nandakumar, K. S., Johansson, B. P., Björck, L., & Holmdahl, R. (2007). Blocking of experimental arthritis by cleavage of IgG antibodies in vivo. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology, 56(10), 3253-3260.
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55753/1472_C7_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cartilage
Immunohistochemical staining of human cartilage shows moderate cytoplasmic positivity in the chondrocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/2214/1552_866_400x400.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2214/169753_B_7_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, HAP1, HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139219-COL2A1
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Specifications
- Immunogen
- Mouse collagen, type II, alpha 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Mouse J1
- Species Reactivity
- Mouse
- Clone
- M2139
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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