Recombinant Mouse Anti-Mouse J1 Antibody (M2139) (CAT#: PABL-649)
Recombinant Mouse Antibody (M2139) is capable of binding to Mouse J1 , expressed in Chinese Hamster Ovary cells (CHO). This mAb specifically recognizes the J1 epitope and is the most arthritogenic anti-CII mAb in the mouse, eliciting disease upon single transfer.
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Figure 1 Western blot analysis antibody M2139 binding to recombinant IdeS.
Recombinant IdeS was incubated with purified monoclonal antibody (mAb) M287 (IgG2a), CIIC1 (IgG2a), M2139 (IgG2b), M284 (IgG1), or B10 (IgG3), separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis under reducing conditions on Precise Protein Gradient Gels, and stained with Coomassie blue.
Nandakumar, K. S., Johansson, B. P., Björck, L., & Holmdahl, R. (2007). Blocking of experimental arthritis by cleavage of IgG antibodies in vivo. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology, 56(10), 3253-3260.
Specifications
- Immunogen
- Mouse collagen, type II, alpha 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Mouse J1
- Species Reactivity
- Mouse
- Clone
- M2139
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- COL2A1; collagen, type II, alpha 1; Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100856; Rgsc856; collagen alpha-1(II) chain; chondrocalcin; alpha-1 type II collagen; disproportionate micromelia; procollagen, type II, alpha 1
- Gene ID
- 12824
- UniProt ID
- P28481
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-225 | Recombinant Anti-human COL2A1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-225 | Recombinant Human Anti-human COL2A1 Antibody | WB, FC, FuncS | IgG |
| HPAB-0281-YC | Human Anti-COL2A1 Recombinant Antibody (HPAB-0281-YC) | ELISA, IHC, FuncS | Human IgG |
| PABC-248 | Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1) | ELISA, IHC | Mouse IgG |
| ZG-0173J | Mouse Anti-COL2A1 Recombinant Antibody (clone 1H1) | IHC-P, IF | Mouse IgG |
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