Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23) (CAT#: HPAB-0282-YC)
The NOTCH signaling pathway is a universally conserved signal transduction system. It is involved in cell fate determination during development including embryonic pattern formation and post-embryonic tissue maintenance. In addition, NOTCH signaling has been identified as a critical factor in the maintenance of hematopoietic stem cells. The product is a novel antibody which binds to NOTCH3.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
Specifications
- Immunogen
- Recombinant human NOTCH3 protein amino acids 350-580 (NOTCH3 EGFs 9-14)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NOTCH3
- Species Reactivity
- Human
- Clone
- 122M23
- Applications
- ELISA, IHC, WB
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- NOTCH3; notch 3; IMF2; CASIL; CADASIL; neurogenic locus notch homolog protein 3; Notch homolog 3
- Gene ID
- 4854
- UniProt ID
- Q9UM47
Related Resources
Breast Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1929z | Mouse Anti-NOTCH3 Recombinant Antibody (clone 22H2) | WB, ELISA | Mouse IgG2a |
| HPAB-1010WJ | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12) | ELISA, WB, FC | Human IgG |
| HPAB-1011WJ | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160) | ELISA, WB, FC | Human IgG |
| HPAB-1012WJ | Human Anti-NOTCH3 Recombinant Antibody (clone N3LHCS_18) | ELISA, WB, FC | Human IgG |
| VS3-QX826 | Mouse Anti-NOTCH3 Recombinant Antibody (clone 487CT6.9.2) | WB | Mouse IgG1 |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-557MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-557MZ) | Inhib, Neut | Chimeric (mouse/human) IgG1 |
| TAB-557MZ-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-557MZ-S(P)) | ELISA, WB | Mouse scFv |
| TAB-557MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-557MZ-F(E)) | ELISA, WB | Chimeric (mouse/human) Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-558MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-558MZ) | Inhib, Neut | Humanized IgG1 |
| TAB-558MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-558MZ-S(P)) | ELISA, WB | Humanized scFv |
| TAB-558MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-558MZ-F(E)) | ELISA, WB | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-559MZ | Anti-Human NOTCH3 Recombinant Antibody (59R1) | FC, Inhib, FuncS | Human antibody |
| TAB-560MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-560MZ) | FC, FACS, FuncS | Human IgG1, κ |
| TAB-561MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-561MZ) | FC | Human IgG |
| TAB-554MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-554MZ-S(P)) | ELISA | Human scFv |
| TAB-555MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-555MZ-S(P)) | ELISA | Human scFv |
Mouse Antibody
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0754-WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone N37); Fab Fragment | ELISA | Human Fab |
| HPAB-1010WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-1011WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-1012WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone N3LHCS_18); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-1013WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone N1wc561); Fab Fragment | ELISA, WB, FC | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0754-WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N37); scFv Fragment | ELISA | Human scFv |
| HPAB-1010WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1011WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1012WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N3LHCS_18); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1013WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N1wc561); scFv Fragment | ELISA, WB, FC | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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