Afuco™ Anti-Human TLR4 ADCC Recombinant Antibody (NI-0101), ADCC Enhanced
CAT#: AFC-591CL
Anti-TLR4 ADCC Enhanced Antibody (NI-0101) is an ADCC enhanced antibody produced by our Afuco™ platform. NI-0101 is an anti-Toll-like Receptor 4 (TLR4) monoclonal antibody that can be used in various chronic inflammatory diseases including, arthritis, atherosclerosis, asthma, lupus, osteoporosis, transplant rejection, and contact dermatitis.
Published Data
Gene Expression
BL
Figure 1 Interference in toll-like receptor 4 (TLR4) signaling blocks IL-6, TNFα, IL-1β and IL-8 production by monocytes stimulated by synovial fluid from patients with rheumatoid arthritis (RASF).
LR4 signaling was blocked with anti-human TLR4 monoclonal antibody, NI-0101. Representative data shown for monocytes obtained from one of seven patients with rheumatoid arthritis (RA).
Hatterer, E., Shang, L., Simonet, P., Herren, S., Daubeuf, B., Teixeira, S.,... & Sokolove, J. (2016). A specific anti-citrullinated protein antibody profile identifies a group of rheumatoid arthritis patients with a toll-like receptor 4-mediated disease. Arthritis research & therapy, 18(1), 224.
FuncS
Figure 2 Heterogeneous capacity of rheumatoid arthritis (RA) synovial fluid (RASF) samples to stimulate cytokine production and respond to TLR4 blockade.
RASF samples from patients (Pat) were classified as NI-0101 responders if NI-0101 was able to block (partially or totally) RASF-induced IL-6 production from RA monocytes. Others were classified as NI-0101 non-responders. a, b Representative examples of RASF from non-responders (Pat#13, Pat#35) and from responders (Pat#27, Pat#18). Of the 40 RASF samples tested, 20 were classified as NI-0101 responders (50 %) and 20 as NI-0101 non-responders (50 %). c, d Mean levels of IL-6 in RASF NI-0101 responder and non-responder RASF samples. IL-6 is presented as normalized values with IL-6 levels induced by RASF arbitrarily set as 100 % induction. RASF samples that were unable to induce IL-6 from RA monocytes but were impacted upon by NI-0101 are excluded from the non-responder group.
Hatterer, E., Shang, L., Simonet, P., Herren, S., Daubeuf, B., Teixeira, S.,... & Sokolove, J. (2016). A specific anti-citrullinated protein antibody profile identifies a group of rheumatoid arthritis patients with a toll-like receptor 4-mediated disease. Arthritis research & therapy, 18(1), 224.
FuncS
Figure 3 Expression of anti-citrullinated protein antibodies (ACPA) and ligands of toll-like receptor 4 in samples of synovial fluid (SF) from patients with rheumatoid arthritis (RA) and correlation between these and NI-0101 response.
a Expression of ACPA, HMGB1 and S100A8/A9 in SF from subjects without RA (non-RA SF) (n = 4 samples) and patients with RA (RASF) (n = 40 samples). b Correlation between ACPA, HMGB1 or S100A8/A9 and NI-0101 response. RASF samples were classified as NI-0101 non-responders (NR) or NI-0101 responders (R) according to Fig. 2.
Hatterer, E., Shang, L., Simonet, P., Herren, S., Daubeuf, B., Teixeira, S.,... & Sokolove, J. (2016). A specific anti-citrullinated protein antibody profile identifies a group of rheumatoid arthritis patients with a toll-like receptor 4-mediated disease. Arthritis research & therapy, 18(1), 224.
Figure 4 Anti-citrullinated protein antibodies (ACPA) fine specificity in the synovial fluid samples of patients with rheumatoid arthritis and their correlation with NI-0101 response.
Antibody reactivity against the citrullinated peptides derived from fibrinogen-α (cFbα − pept), fibrinogen-β (cFbβ-pept) and histone-2A (cH2A-pept) were determined by ELISA and expression in synovial fluids was measured in ACPA-positive rheumatoid arthritis synovial fluid (RASF) samples (a) (n = 22) and both ACPA-negative and ACPA-positive RASF samples (b) (n = 40). RASF samples were classified as NI-0101 non-responders (NR) or NI-0101 responders (R).
Hatterer, E., Shang, L., Simonet, P., Herren, S., Daubeuf, B., Teixeira, S.,... & Sokolove, J. (2016). A specific anti-citrullinated protein antibody profile identifies a group of rheumatoid arthritis patients with a toll-like receptor 4-mediated disease. Arthritis research & therapy, 18(1), 224.
Figure 5 Anti-citrullinated protein antibodies (ACPA) fine specificity in paired sera samples of patients with rheumatoid arthritis (RA) and their correlation with RA synovial fluid (RASF) response to NI-0101.
a Correlation between ACPA in paired samples of sera from patients with RA and synovial fluids (n = 22). b ACPA in paired samples of sera from patients with RA classified according to RASF response to NI-0101 (NI-0101 non-responders (NR) or NI-0101 responders (R)). c, d Antibody reactivity against the citrullinated peptides derived from fibrinogen-α (cFbα-pept), fibrinogen-β (cFbβ-pept) and histone-2A (cH2A-pept) were determined by ELISA in paired samples of sera from patients with RA and correlation with response to NI-0101 was tested. c Paired sera samples from ACPA-positive patients with RA (n = 13). d Paired samples of sera from from ACPA-positive and ACPA-negative patients with RA (n = 22).
Hatterer, E., Shang, L., Simonet, P., Herren, S., Daubeuf, B., Teixeira, S.,... & Sokolove, J. (2016). A specific anti-citrullinated protein antibody profile identifies a group of rheumatoid arthritis patients with a toll-like receptor 4-mediated disease. Arthritis research & therapy, 18(1), 224.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/912_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows distinct cytoplasmic positivity in subsets of cells in the red pulp.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/911_G5_4_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49174/140817_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Karpas-707, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136869-TLR4
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Specifications
- Host Species
- Humanized
- Derivation
- Humanized
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- FuncS
- Related Disease
- Inflammatory Diseases
Product Property
- Purity
- >95% by HPLC
- Storage
- Short term: store at 4°C (over 6 months), long term: -20°C or -80°C.
Applications
- Application Notes
- The antibody was validated for Function Assay. For details, refer to Published Data.
Target
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Related Signaling Pathways
NLR Signaling Pathway
TLR Signaling Pathway
Related Diseases
Rheumatoid Arthritis
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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