Anti-Human TNFRSF17 Therapeutic Antibody (13C2) (CAT#: TAB-1208CL)

The anti-BCMA antibody specifically binds to human BCMA which is involved in B cell maturation, growth and survival. The antibody can be used in the treatment of lymphomas and multiple myeloma.

  • Published Data
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 Binding of anti-BCMA antibodies on BCMA-positive multiple myeloma cells.

Mean fluorescence intensity for anti-BCMA IgG clones plotted in function of anti-BCMA antibody concentrations (from 0.2 to 40 μg/mL); (A) clones 13C2, 17A5, 83A10 on H929 cells, (B) clones 13C2, 30 17A5, 83A10 on MKN45 cells.

ELISA

Figure 2 Competition ELISA.

ELISA results of 7 selected anti-BCMA Fab clones (13C2, 17A5, 83A19, 13A4, 13D2, 29B 11, 13A7), at saturating concentrations of 500 or 1000 nM, binding to immobilized human BCMA in the presence of a concentration range of murine APRIL (from 1.56 to 100 nM) are shown. In case of non-competition, signals remain constant within the variability of the assay across the concentration range and signals in the presence of murine APRIL are comparable to those from the control wells where no murine APRIL was added. In case of competition a concentration dependent reduction of the signal is measured.

FC

Figure 3 Binding competition by FACS.

Competition of Δ-APRIL with anti-BCMA antibodies detected by flow cytometry. Relative median fluorescence intensity of Δ-APRIL (FITC signal) used at a concentration of 1000 ng/mL detected in function of concentrations (1, 16, and 40 μg/mL) of anti- BCMA antibody clones 13A4, 13D2, 14E1, 14B 11 on H929 cells. The median fluorescence intensity upon binding of Δ-APRIL in presence of the isotype control was set to one; the other signals were normalized to it. The detection of APRIL binding to BCMA-positive H929 cells in the presence of anti- BCMA antibodies was measured via anti-HA fluorochrome-conjugated antibody.

FC

Figure 4 Binding competition by FACS.

Competition of anti-BCMA antibodies with Δ-APRIL detected by flow cytometry. The relative median fluorescence intensity of anti-BCMA antibody (Alexa.Fluor 647 signal) used at a concentration of 40 μg/mL for anti-BCMA antibody clones 13A4, 13C7, 13D2, 14B 11, 17A5, 83A10 on RPMI cells detected in absence or presence of Δ-APRIL 1000 ng/mL. The median fluorescence intensity upon binding of anti-BCMA antibodies in absence of Δ-APRIL was set to one; the other signals respective to the anti-BCMA antibody in presence of Δ-APRIL were normalized to it. The detection of anti-BCMA antibodies binding to BCMA-positive RPMI cells in the presence of Δ- APRIL was measured via anti-human Fc fluorochrome-conjugated antibody.


Specifications

  • Immunogen
  • BCMA-ECD-biot
  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • Human
  • Clone
  • 13C2
  • Applications
  • ELISA, FC
  • Related Disease
  • Lymphomas, Multiple myeloma

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Flow Cytometry.

Target

  • Alternative Names
  • TNFRSF17; tumor necrosis factor receptor superfamily, member 17; BCM; BCMA; CD269; TNFRSF13A; tumor necrosis factor receptor superfamily member 17; B-cell maturation factor; B cell maturation antigen; B-cell maturation protein;

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "TNFRSF17"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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