Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) (CAT#: HPAB-0333-YC)

Figure 1 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in ELISA

ELISA analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag). Then blocked with BSA and incubated with HPAB-0333-YC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.6 μg)

Figure 2 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in WB

Western blot analysis of HPAB-0333-YC was performed with Human CD30/TNFRSF8 Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0333-YC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 3 Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) in DB

Dot Blot analysis of HPAB-0333-YC was performed by coating with Human CD30/TNFRSF8 Protein (His Tag).

HPAB-0333-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control