Recombinant Human Anti-VEGF Antibody (Fab-12) (CAT#: PABX-185)
Anti-VEGF antibody is a Chimeric antibody (mouse/human) of IgG1 κ class that binds to an VEGF.
Specific Inquiry
Figure 1 The effects of VEGF Trap, ranibizumab and bevacizumab on calcium mobilization induced byVEGF-A165 in HUVEC.
Serial dilutions of VEGF Trap (open box), ranibizumab (triangle) or bevacizumab (closed circle) were added to HUVEC along with 20 pM of VEGF-A165. The VEGF-A165 was preincubated with the inhibitors for 10 min at 25 C. The solution was added to HUVEC preloaded with fluo-4 and the fluorescence of the well was determined on a FLIPR instrument. The data were plotted using a four parameter curve fit with GraphPad Prism. Each point represents duplicate wells at each concentration.
Papadopoulos, N., Martin, J., Ruan, Q., Rafique, A., Rosconi, M. P., Shi, E., ... & Wiegand, S. J. (2012). Binding and neutralization of vascular endothelial growth factor (VEGF) and related ligands by VEGF Trap, ranibizumab and bevacizumab. Angiogenesis, 15(2), 171-185.
Figure 2 Radiolabeled VEGF binding assay
[125I]VEGF was equilibrated (23 °C) with serial dilutions of unlabeled VEGF and (a) Fab-12 or (c) Y0317. Fabs were captured with an anti-Fab antibody-coated immunosorbant plate. Scatchard analysis (Munson & Rodbard, 1980) with a 1:1 binding model was used to calculate Kd of (b) 433 (116) pM for Fab-12 and (d) 19.8(4.3) pM for Y0317.
Clark, L. A., Boriack-Sjodin, P. A., Day, E., Eldredge, J., Fitch, C., Jarpe, M., ... & Van Vlijmen, H. W. (2008). An antibody loop replacement design feasibility study and a loop-swapped dimer structure. Protein Engineering, Design & Selection, 22(2), 93-101.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human VEGF
- Species Reactivity
- Human
- Clone
- Fab-12
- Applications
- ELISA Assay-Dependent
FuncS Assay-Dependent
Block Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody Fab-12 has been reported in applications of ELISA, FuncS, Block. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- VEGF; Vascular endothelial growth factor; EPI0030; BD0801; Sevacizumab
Related Resources
Bladder Cancer
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
scFv Fragment Antibody
Chimeric Antibody
Mouse Antibody
Human Antibody
Humanized Antibody
Fc Glycosylation
Deglycosylated Antibody (Non-glycosylated IgGs)
Rabbit Monoclonal Antibody
Fab Fragment Antibody
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1036 | Recombinant Anti-VEGF Antibody | ELISA, WB, Dot, FuncS | IgG |
| HPAB-2709LY | Human Anti-VEGF Recombinant Antibody (clone YS03) | ELISA | Human IgG |
| HPAB-AP213-YC | Human Anti-VEGF Recombinant Antibody (HPAB-AP213-YC) | ELISA | Human IgG |
| HPAB-2806LY | Human Anti-VEGF Recombinant Antibody (HPAB-2806LY) | WB, FuncS | Humanized IgG |
| HPAB-2807LY | Human Anti-VEGF Recombinant Antibody (HPAB-2807LY) | ELISA, WB | Humanized IgG |
Customer Reviews and Q&As
Customer Review
Q&As
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Great for VEGF-related experiments
The Recombinant Human Anti-VEGF Antibody (Fab-12) has significantly improved the accuracy of our angiogenesis assays. Its high affinity and specificity make it indispensable for our VEGF-related experiments. Highly recommended! -
Helpful for studies on diabetic retinopathy
We are using this antibody for in vitro studies on diabetic retinopathy. The specificity and inhibition of the VEGF pathways we've observed have been highly insightful. This antibody is fundamental to our project -
High affinity
The Recombinant Human Anti-VEGF Antibody (Fab-12) stands out for its high affinity, versatility in applications, and robust performance in both in vitro and in vivo models. It offers superior specificity and sensitivity compared to many commercially available VEGF antibodies.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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