What does the Anti-VEGF Antibody do?

The Anti-VEGF Antibody Fab Fragment helps researchers study the VEGF protein, important for blood vessel formation. It's commonly used in ELISA and blocking assays to explore how VEGF works in diseases like cancer, enhancing our understanding of this vital protein.

What applications is this antibody suited for?

It's ideal for use in ELISA, functional studies, and blocking assays. Researchers can rely on its high purity and specificity to gather detailed data on VEGF functions.

How do I store this antibody properly?

You should store the antibody in small portions at -20°C for the best stability. This ensures it stays effective over time, making sure your experiments continue to produce reliable results.

Recombinant Mouse Anti-VEGF Antibody Fab Fragment (Fab-12)

CAT#: PABX-185-F (E)

Anti-VEGF antibody is a Mouse of Fab class that binds to an VEGF.

Published Data

Figure 1 Binding specificities and receptor blocking activities of the phage-derived antibodies.

A, sequences of four different phage clones initially identified were compared with template h4D5. Shaded residues were not randomized in the antibody libraries. The relative affinities for h-VEGF and m-VEGF were measured with competitive ELISA (EC50). The receptor blocking activities of the four clones were screened by measuring phage binding to m-VEGF-coated wells in the presence of either serial dilutions of VEGFR2 ECD (VEGFR2D1–7) (B) or second Ig domain of VEGFR1 ECD (VEGFR1D2) (C). D, binding specificities of G6 or B20-4 Fabs were measured by binding to h-VEGF (VEGF-A)-, m-VEGF-, h-PlGF-, m-PlGF-, m-VEGF-D-, or h-VEGF-B-coated wells, and bound Fab was detected. E, receptor blocking of Fab was confirmed by the binding of biotinylated m-VEGF to immobilized VEGFR2D1–7 in the presence of increasing concentration of G6, B20-4, Fab-12 (Fab of Avastin), or Y0317.

Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.

❮

❯

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Human

Type
Fab

Antibody Isotype
IgG1 κ

Specificity
Tested positive against native Human VEGF

Species Reactivity
Human

Clone
Fab-12

Applications
ELISA Assay-Dependent
FuncS Assay-Dependent
Block Assay-Dependent

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
The antibody Fab-12 Fab fragment has been reported in applications of ELISA, FuncS, Block. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

Alternative Names
VEGF; Vascular endothelial growth factor; EPI0030; BD0801; Sevacizumab

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for PABX-185-F (E). Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

High Sensitivity Levels

The sensitivity of this fab fragment is outstanding, evident in our ELISA assays. Its ability to detect subtle variations in VEGF levels makes it a powerful tool in our research arsenal.

Excellent for Cancer Studies

As part of our cancer research investigations, this antibody has been pivotal. It helps us understand how tumors promote blood vessel growth, offering insights into potential therapeutic targets.

Q&As

What does the Anti-VEGF Antibody do?

A: The Anti-VEGF Antibody Fab Fragment helps researchers study the VEGF protein, important for blood vessel formation. It's commonly used in ELISA and blocking assays to explore how VEGF works in diseases like cancer, enhancing our understanding of this vital protein.
What applications is this antibody suited for?

A: It's ideal for use in ELISA, functional studies, and blocking assays. Researchers can rely on its high purity and specificity to gather detailed data on VEGF functions.
How do I store this antibody properly?

A: You should store the antibody in small portions at -20°C for the best stability. This ensures it stays effective over time, making sure your experiments continue to produce reliable results.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

Bladder Cancer

Rheumatoid Arthritis

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-185-F (E).pdf

MSDS

MSDS - PABX-185-F (E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone Fab-12"

CAT
Product Name

See other products for "VEGF"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1036	Recombinant Anti-VEGF Antibody	ELISA, WB, Dot, FuncS	IgG

CAT	Product Name	Application	Type
MOB-1036-F(E)	Recombinant Anti-VEGF Antibody Fab Fragment	RIA, IF, FuncS	Fab

CAT	Product Name	Application	Type
MOB-1036-S(P)	Recombinant Anti-VEGF Antibody scFv Fragment	ELISA, WB, IP, FuncS	scFv

CAT	Product Name	Application	Type
MHH-1036	Recombinant Human Anti-VEGF Antibody	ELISA, WB, FC, FuncS	IgG

CAT	Product Name	Application	Type
MHH-1036-F(E)	Recombinant Human Anti-VEGF Antibody Fab Fragment	ELISA, IF, FuncS	Fab

CAT	Product Name	Application	Type
MHH-1036-S(P)	Recombinant Human Anti-VEGF Antibody scFv Fragment	ELISA, IP, FuncS	scFv

CAT	Product Name	Application	Type
TAB-658CL	Human Anti-VEGF Recombinant Antibody (TAB-658CL)	ELISA	Human IgG

CAT	Product Name	Application	Type
PABZ-130	Mouse Anti-VEGF Recombinant Antibody (clone 12)	ELISA, Neut	Mouse IgG

CAT	Product Name	Application	Type
PABW-130	Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1)	Neut, ELISA, Inhib, ICC, WB	Mouse IgG

CAT	Product Name	Application	Type
PABC-131	Human Anti-VEGF Recombinant Antibody (clone B20-4)	ELISA, Neut, Block	Human IgG

CAT	Product Name	Application	Type
PFBZ-130	Mouse Anti-VEGF Recombinant Antibody (clone 12); Fab Fragment	ELISA, Neut	Mouse Fab

CAT	Product Name	Application	Type
PFBW-130	Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1); Fab Fragment	Neut, ELISA	Mouse Fab

CAT	Product Name	Application	Type
PFBC-131	Human Anti-VEGF Recombinant Antibody (clone B20-4); Fab Fragment	ELISA, Neut, Block	Human Fab

CAT	Product Name	Application	Type
PSBZ-130	Mouse Anti-VEGF Recombinant Antibody (clone 12); scFv Fragment	ELISA, Neut	Mouse scFv

CAT	Product Name	Application	Type
PSBW-130	Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1); scFv Fragment	Neut, ELISA	Mouse scFv

CAT	Product Name	Application	Type
PSBC-131	Human Anti-VEGF Recombinant Antibody (clone B20-4); scFv Fragment	ELISA, Neut, Block	Human scFv

CAT	Product Name	Application	Type
TAB-0345CL	Anti-Human VEGF Recombinant Antibody (4D3)	ELISA, FuncS, Inhib

CAT	Product Name	Application	Type
TAB-0346CL	Anti-Human VEGF Recombinant Antibody (FD006)	ELISA, PK, Inhib, IHC, WB	Human antibody

CAT	Product Name	Application	Type
TAB-0345CL-S(P)	Anti-Human VEGF Recombinant Antibody scFv Fragment (4D3)	ELISA, FuncS, Inhib

CAT	Product Name	Application	Type
TAB-0346CL-S(P)	Anti-Human VEGF Recombinant Antibody scFv Fragment (FD006)	ELISA, FuncS, Inhib	Human antibody

CAT	Product Name	Application	Type
TAB-0345CL-F(E)	Anti-Human VEGF Recombinant Antibody Fab Fragment (4D3)	ELISA, FuncS, Inhib

CAT	Product Name	Application	Type
TAB-285CQ	Mouse Anti-VEGF Recombinant Antibody (TAB-285CQ)	ELISA, WB, Inhib	Mouse IgG1

CAT	Product Name	Application	Type
TAB-287CQ	Mouse Anti-VEGF Recombinant Antibody (TAB-287CQ)	ELISA	Mouse IgG1, κ

CAT	Product Name	Application	Type
TAB-288CQ	Human Anti-VEGF Recombinant Antibody (TAB-288CQ)	ELISA	Chimeric (Mouse/Human) IgG1, κ

CAT	Product Name	Application	Type
TAB-289CQ	Human Anti-VEGF Recombinant Antibody (TAB-289CQ)	ELISA	Human IgG1, κ

CAT	Product Name	Application	Type
TAB-290CQ	Anti-Human VEGF Recombinant Antibody	ELISA, WB, Neut

CAT	Product Name	Application	Type
TAB-293CQ	Anti-Human VEGF Recombinant Antibody (HAB1)	WB	Humanized antibody

CAT	Product Name	Application	Type
TAB-294CQ	Anti-Human VEGF Recombinant Antibody (hAB2)	ELISA, Inhib	Humanized antibody

CAT	Product Name	Application	Type
TAB-295CQ	Anti-Human VEGF Recombinant Antibody (hAB3)	ELISA, Inhib	Humanized antibody

CAT	Product Name	Application	Type
TAB-301CQ	Human Anti-VEGF Recombinant Antibody (TAB-301CQ)	ELISA, FC, IHC, WB	Chimeric (Mouse/Human) IgG1, κ

CAT	Product Name	Application	Type
TAB-303CQ	Anti-Human VEGF Recombinant Antibody (TAB-303CQ)	ELISA, WB, Neut

CAT	Product Name	Application	Type
TAB-285CQ-S(P)	Mouse Anti-VEGF Recombinant Antibody; scFv Fragment (TAB-285CQ-S(P))	ELISA, WB, Neut	Mouse scFv

CAT	Product Name	Application	Type
TAB-288CQ-S(P)	Human Anti-VEGF Recombinant Antibody; scFv Fragment (TAB-288CQ-S(P))	ELISA, WB, IHC, Block	Human scFv

CAT	Product Name	Application	Type
TAB-301CQ-S(P)	Human Anti-VEGF Recombinant Antibody; scFv Fragment (TAB-301CQ-S(P))	ELISA, FC, IHC, WB	Human scFv

CAT	Product Name	Application	Type
TAB-288CQ-F(E)	Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-288CQ-F(E))	ELISA, WB, IHC, Block	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
Gly-096LC	Recombinant Anti-Human VEGF Antibody (Fc glycosylation)	ELISA	Humanized antibody
Gly-143LC	Recombinant Anti-Human VEGF Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-179LC	Recombinant Anti-Human VEGF Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
MOR-4485	Rabbit Anti-VEGF Recombinant Antibody (clone SI581DS)	ELISA	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-J0267-YC	Recombinant Camelid Anti-VEGF Single Domain Antibody (HPAB-J0267-YC)	ELISA, Neut	Camelid VHH

CAT	Product Name	Application	Type
HPAB-J0268-YC	Recombinant Camelid Anti-VEGF Single Domain Antibody (HPAB-J0268-YC)	ELISA, Neut	Camelid VHH

CAT	Product Name	Application	Type
VS-0825-YC353	SmartAb™ Recombinant Anti-VEGF pH-dependent Antibody (Clone 4D3)	ELISA, Inhib	Human IgG

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 5 Anti-Human CD40L Recombinant Antibody (TAB-027) in ELISA

Anti-Human CD40L Recombinant Antibody (TAB-027) (CAT# TAB-027)
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC

Figure 3 Anti-Human Abeta Antibody in DB

Anti-Human Abeta Recombinant Antibody (TAB-229) (CAT# TAB-229)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC

Figure 2 Anti-Human NGF Recombinant Antibody (TAB-111) in ELISA

Anti-Human NGF Recombinant Antibody (TAB-111) (CAT# TAB-111)
Application: ELISA, FuncS, Inhib

Figure 3 Anti-CD40 Antibody (TAB-126CL) in Dot Blot

Human Anti-CD40 Recombinant Antibody (TAB-126CL) (CAT# TAB-126CL)
Application: Block, Cyt, FuncS, Inhib

Figure 4 Anti-Human EPCAM Recombinant Antibody (TAB-235CL) in ELISA

Human Anti-Human EPCAM Recombinant Antibody (3622W94) (CAT# TAB-235CL)
Application: Cyt, FC

Figure 1 Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) in SDS-PAGE

Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) (CAT# PABL-202)
Application: WB, ELISA, FuncS

Figure 5 Mouse Anti-His Tag Recombinant Antibody Fab Fragment (PFBL-134) in HPLC

Mouse Anti-His Tag Recombinant Antibody (clone YC103); Fab Fragment (CAT# PFBL-134)
Application: WB, ELISA, FuncS

Figure 2 Anti-NCR3LG1 Recombinant Antibody (TAB-1134CL) in SEC-HPLC

Mouse Anti-NCR3LG1 Recombinant Antibody (TAB-1134CL) (CAT# TAB-1134CL)
Application: ELISA

Figure 4 Anti-Human CD59 Recombinant Antibody Fab Fragment (TAB-321LC-F(E)) in DB

Anti-Human CD59 Recombinant Antibody Fab Fragment ((ch)AR36A36.11.1) (CAT# TAB-321LC-F(E))
Application: WB, ELISA

Figure 2 Anti-FGFR1 Therapeutic Antibody (FR1-H7) (TAB-052WM) in ELISA

Anti-Human FGFR1 Recombinant Antibody (FR1-H7) (CAT# TAB-052WM)
Application: ELISA, Neut

Figure 5 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in ELISA

Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) (CAT# TAB-168CQ)
Application: ELISA, FC, WB, FuncS

Figure 5 Anti-human IL23A Recombinant antibody (TAB-620LC) in HPLC

Anti-human IL23A Recombinant Antibody (TAB-620LC) (CAT# TAB-620LC)
Application: ELISA

Figure 1 Anti-CD20 Antibody (PABX-028) in Flow Cytometry

Recombinant Human Anti-CD20 Antibody (PABX-028) (CAT# PABX-028)
Application: WB, ELISA, IF, FuncS, Apop, BA

Figure 5 Anti-Canine PDCD1 Recombinant Antibody (TAB-028ML) in ELISA

Anti-Canine PDCD1 Recombinant Antibody (TAB-028ML) (CAT# TAB-028ML)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 1 Anti-Human RSPO3 Recombinant Antibody (TAB-449CQ) in SDS-PAGE

Anti-Human RSPO3 Recombinant Antibody (Rosmantuzumab) (CAT# TAB-449CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 3 Anti-Human F3 Antibody (TAB-473CQ) in ELISA

Anti-Human F3 Recombinant Antibody (TAB-473CQ) (CAT# TAB-473CQ)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 1 Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) in SDS-PAGE

Mouse Anti-ROR1 Recombinant Antibody (HPAB-0308-CN) (CAT# HPAB-0308-CN)
Application: ELISA, FC

Figure 4 Human Anti-TGFBR2 Recombinant Antibody (HPAB-0150-FY) in DB

Human Anti-TGFBR2 Recombinant Antibody (clone LY3022859) (CAT# HPAB-0150-FY)
Application: ELISA

Figure 3 Human Anti-IL21R Recombinant Antibody (FAMAB-1203CQ) in DB

Human Anti-IL21R Recombinant Antibody (clone ATR-107) (CAT# FAMAB-1203CQ)
Application: Inhib, FC, WB

Figure 5 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in DB

Mouse Anti-Fc P329G LALA Recombinant Antibody (clone P329G LALA) (CAT# PABJ-0115)
Application: ELISA, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.