Recombinant Mouse Anti-VEGF Antibody Fab Fragment (Fab-12) (CAT#: PABX-185-F (E))
Anti-VEGF antibody is a Mouse of Fab class that binds to an VEGF.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
-1.png)
Figure 1 Binding specificities and receptor blocking activities of the phage-derived antibodies.
A, sequences of four different phage clones initially identified were compared with template h4D5. Shaded residues were not randomized in the antibody libraries. The relative affinities for h-VEGF and m-VEGF were measured with competitive ELISA (EC50). The receptor blocking activities of the four clones were screened by measuring phage binding to m-VEGF-coated wells in the presence of either serial dilutions of VEGFR2 ECD (VEGFR2D1–7) (B) or second Ig domain of VEGFR1 ECD (VEGFR1D2) (C). D, binding specificities of G6 or B20-4 Fabs were measured by binding to h-VEGF (VEGF-A)-, m-VEGF-, h-PlGF-, m-PlGF-, m-VEGF-D-, or h-VEGF-B-coated wells, and bound Fab was detected. E, receptor blocking of Fab was confirmed by the binding of biotinylated m-VEGF to immobilized VEGFR2D1–7 in the presence of increasing concentration of G6, B20-4, Fab-12 (Fab of Avastin), or Y0317.
Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native Human VEGF
- Species Reactivity
- Human
- Clone
- Fab-12
- Applications
- ELISA Assay-Dependent
FuncS Assay-Dependent
Block Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody Fab-12 Fab fragment has been reported in applications of ELISA, FuncS, Block. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- VEGF; Vascular endothelial growth factor; EPI0030; BD0801; Sevacizumab
Related Resources
Bladder Cancer
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone Fab-12"
See other products for "VEGF"
Select a product category from the dropdown menu below to view related products.
Please select product type
ScFv Antibody Products
Human Antibody Products
Mouse Antibody Products
Chimeric Antibody Products
Humanized Antibody Products
Fc Glycosylation Antibody Products
Deglycosylated Antibody Products
Rabbit Monoclonal Antibody Products
Fab Antibody Products
IgG Antibody Products
Single Domain Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1036-S(P) | Recombinant Human Anti-VEGF Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-0746LY-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-0746LY-S(P)) | ELISA | Humanized scfv |
| HPAB-2709LY-S(P) | Human Anti-VEGF Recombinant Antibody (clone YS03); scfv Fragment | ELISA | Human scfv |
| HPAB-2813LY-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-2813LY-S(P)) | ELISA, WB | Humanized scfv |
| HPAB-2814LY-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-2814LY-S(P)) | ELISA, WB | Humanized scfv |
Customer Reviews and Q&As
Customer Review
Q&As
High Sensitivity Levels
The sensitivity of this fab fragment is outstanding, evident in our ELISA assays. Its ability to detect subtle variations in VEGF levels makes it a powerful tool in our research arsenal.
Excellent for Cancer Studies
As part of our cancer research investigations, this antibody has been pivotal. It helps us understand how tumors promote blood vessel growth, offering insights into potential therapeutic targets.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-3.png)
Application: ELISA, FC, Block, SPR
-2-1.png)
Application: ELISA
Application: Neut, FC
Application: WB, IHC, ELISA, FC
Application: ELISA, IHC, IF, IP, FC, FuncS
Application: ELISA, FC, Neut, Inhib
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
