Recombinant Mouse Anti-VEGF Antibody scFv Fragment (YADS1)
CAT#: PABX-187-S (P)
Anti-VEGF antibody is a Mouse of scFv class that binds to an VEGF.
Published Data
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Figure 1 Surface plasmon resonance (SPR) analysis
We reasoned that YADS2 and YADS3 recognized VEGF through a very similar mechanism, as they exhibited high sequence homology in their CDRs and bound to both human and murine VEGF. In contrast, YADS1 likely represented a unique mode of antigen recognition as it contained very different CDR sequences and did not recognize mVEGF.
-2.png)
Figure 2 Thermodynamic characterization of the interactions between hVEGF and Fabs.
Isothermal titration calorimetry of (a) Fab-YADS1 and (b) Fab-YADS2 binding to hVEGF at 25 8C. In each panel, the rates of heat release upon binding are in the top plot and the integrated heats plotted against the molar ratio of Fab are in the bottom plot. Linear regression analysis yielded a molar ratio of 0.94G0.01 or 0.99G0.01 for Fab-YADS1 or Fab-YADS2 binding to hVEGF, respectively.
Fellouse, F. A., Barthelemy, P. A., Kelley, R. F., & Sidhu, S. S. (2006). Tyrosine Plays a Dominant Functional Role in the Paratope of a Synthetic Antibody Derived from a Four Amino Acid Code. Journal of Molecular Biology, 357(1), 100–114.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- scFv
- Specificity
- Tested positive against native Hen Lysozyme
- Species Reactivity
- Human
- Clone
- YADS1
- Applications
- WB 1:1000-1:5000
ELISA Assay-Dependent
Neut Assay-Dependent
FuncS Assay-Dependent
Thermodynamic characterization 3 nM
SPR 3-500 nM
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This YADS1 antibody Fab fragment can be used in applications of WB, ELISA, Neut, FuncS, Thermodynamic characterization, SPR. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Thermodynamic characterization: KD values of Fab-YADS1 measured by ITC at 25°C (3 nM).
SPR: Serial dilutions of Fab proteins (3-500 nM) were injected, and binding responses on the hVEGF or mVEGF flow cell were corrected by subtraction of responses on a blank flow cell.
Target
- Alternative Names
- VEGF; Vascular endothelial growth factor; EPI0030; BD0801; Sevacizumab
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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