Recombinant Mouse Anti-VEGF Antibody scFv Fragment (YADS1) (CAT#: PABX-187-S (P))
Anti-VEGF antibody is a Mouse of scFv class that binds to an VEGF.
Specific Inquiry
-1.png)
Figure 1 Surface plasmon resonance (SPR) analysis
We reasoned that YADS2 and YADS3 recognized VEGF through a very similar mechanism, as they exhibited high sequence homology in their CDRs and bound to both human and murine VEGF. In contrast, YADS1 likely represented a unique mode of antigen recognition as it contained very different CDR sequences and did not recognize mVEGF.
-2.png)
Figure 2 Thermodynamic characterization of the interactions between hVEGF and Fabs.
Isothermal titration calorimetry of (a) Fab-YADS1 and (b) Fab-YADS2 binding to hVEGF at 25 8C. In each panel, the rates of heat release upon binding are in the top plot and the integrated heats plotted against the molar ratio of Fab are in the bottom plot. Linear regression analysis yielded a molar ratio of 0.94G0.01 or 0.99G0.01 for Fab-YADS1 or Fab-YADS2 binding to hVEGF, respectively.
Fellouse, F. A., Barthelemy, P. A., Kelley, R. F., & Sidhu, S. S. (2006). Tyrosine Plays a Dominant Functional Role in the Paratope of a Synthetic Antibody Derived from a Four Amino Acid Code. Journal of Molecular Biology, 357(1), 100–114.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- scFv
- Specificity
- Tested positive against native Hen Lysozyme
- Species Reactivity
- Human
- Clone
- YADS1
- Applications
- WB 1:1000-1:5000
ELISA Assay-Dependent
Neut Assay-Dependent
FuncS Assay-Dependent
Thermodynamic characterization 3 nM
SPR 3-500 nM
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This YADS1 antibody Fab fragment can be used in applications of WB, ELISA, Neut, FuncS, Thermodynamic characterization, SPR. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Thermodynamic characterization: KD values of Fab-YADS1 measured by ITC at 25°C (3 nM).
SPR: Serial dilutions of Fab proteins (3-500 nM) were injected, and binding responses on the hVEGF or mVEGF flow cell were corrected by subtraction of responses on a blank flow cell.
Target
- Alternative Names
- VEGF; Vascular endothelial growth factor; EPI0030; BD0801; Sevacizumab
Related Resources
Bladder Cancer
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1036-F(E) | Recombinant Anti-VEGF Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| PFBZ-130 | Mouse Anti-VEGF Recombinant Antibody (clone 12); Fab Fragment | ELISA, Neut | Mouse Fab |
| PFBW-130 | Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1); Fab Fragment | Neut, ELISA | Mouse Fab |
| PFBC-131 | Human Anti-VEGF Recombinant Antibody (clone B20-4); Fab Fragment | ELISA, Neut, Block | Human Fab |
| HPAB-2709LY-F(E) | Human Anti-VEGF Recombinant Antibody (clone YS03); Fab Fragment | ELISA | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-130 | Mouse Anti-VEGF Recombinant Antibody (clone 12) | ELISA, Neut | Mouse IgG |
| PABW-130 | Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1) | Neut, ELISA, Inhib, ICC, WB | Mouse IgG |
| PABC-131 | Human Anti-VEGF Recombinant Antibody (clone B20-4) | ELISA, Neut, Block | Human IgG |
| HPAB-J0264-YC | Human Anti-VEGF Recombinant Antibody (clone G12) | ELISA | Human IgG |
| HPAB-J0265-YC | Human Anti-VEGF Recombinant Antibody (clone E9) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-285CQ | Mouse Anti-VEGF Recombinant Antibody (TAB-285CQ) | ELISA, WB, Inhib | Mouse IgG1 |
| TAB-287CQ | Mouse Anti-VEGF Recombinant Antibody (TAB-287CQ) | ELISA | Mouse IgG1, κ |
| TAB-290CQ | Anti-Human VEGF Recombinant Antibody | ELISA, WB, Neut | |
| TAB-303CQ | Anti-Human VEGF Recombinant Antibody (EBV321) | ELISA, WB, Neut | |
| PABX-187 | Recombinant Mouse Anti-VEGF Antibody (YADS1) | WB, ELISA, Neut, FuncS | IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-407CQ | Human Anti-VEGF Recombinant Antibody (TAB-407CQ) | ELISA | Humanized IgG |
| TAB-408CQ | Human Anti-VEGF Recombinant Antibody (TAB-408CQ) | ELISA | Humanized IgG |
| TAB-293CQ-S(P) | Anti-Human VEGF Recombinant Antibody scFv Fragment (HAB1) | ELISA, Inhib | Humanized antibody |
| TAB-294CQ-S(P) | Anti-Human VEGF Recombinant Antibody scFv Fragment (hAB2) | ELISA, Inhib | Humanized antibody |
| TAB-295CQ-S(P) | Anti-Human VEGF Recombinant Antibody scFv Fragment (hAB3) | ELISA, Inhib | Humanized antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-288CQ-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (TAB-288CQ-S(P)) | ELISA, WB, IHC, Block | Human scFv |
| TAB-301CQ-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (TAB-301CQ-S(P)) | ELISA, FC, IHC, WB | Human scFv |
| TAB-288CQ-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-288CQ-F(E)) | ELISA, WB, IHC, Block | Chimeric (Mouse/Human) Fab |
| TAB-301CQ-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-301CQ-F(E)) | ELISA, FC, IHC, WB | Chimeric (Mouse/Human) Fab |
| PABX-185-F (E) | Recombinant Mouse Anti-VEGF Antibody Fab Fragment (Fab-12) | ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-296CQ-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (XPA.10.064) | ELISA, IHC, Inhib | Human antibody |
| TAB-297CQ-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (XPA.10.072) | ELISA, IHC, Inhib | Human antibody |
| TAB-298CQ-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-298CQ-F(E)) | ELISA, IF, WB, Inhib | Human Fab |
| TAB-299CQ-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-299CQ-F(E)) | ELISA, IF, WB, Inhib | Human Fab |
| TAB-319CQ-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (TAB-319CQ-F(E)) | ELISA, FC, WB | Human Fab |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-096LC | Recombinant Anti-Human VEGF Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-143LC | Recombinant Anti-Human VEGF Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-179LC | Recombinant Anti-Human VEGF Antibody (Non-glycosylated) | ELISA | Human antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4485 | Rabbit Anti-VEGF Recombinant Antibody (clone SI581DS) | ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0746LY-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-0746LY-S(P)) | ELISA | Humanized scfv |
| HPAB-2817LY-S(P) | Human Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-2817LY-S(P)) | ELISA, WB | Humanized scfv |
| HPAB-J0264-YC-S(P) | Human Anti-VEGF Recombinant Antibody (clone G12); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-J0265-YC-S(P) | Human Anti-VEGF Recombinant Antibody (clone E9); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-J0269-YC-S(P) | Mouse Anti-VEGF Recombinant Antibody (clone EGF165); scFv Fragment | ELISA, WB | Mouse scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0267-YC | Recombinant Camelid Anti-VEGF Single Domain Antibody (V9) | ELISA, Neut | Camelid VHH |
| HPAB-J0268-YC | Recombinant Camelid Anti-VEGF Single Domain Antibody (V93) | ELISA, Neut | Camelid VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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