This recombinant monoclonal antibody is a humanized monoclonal antibody that specifically recognizes human IL-23A and has potent affinity against IL23A. It also can be potentially used in the research of Crohn's disease, psoriasis, psoriatic arthritis and asthma.
Figure 1 Anti-Human IL23A Antibody (TAB-620LC) in WB
Western blot analysis of TAB-620LC was performed by loading human IL23A protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-620LC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
Figure 2 Anti-Human IL23A Antibody (TAB-620LC) in DB
Dot Blot analysis of TAB-620LC was performed by coating with human IL23A protein.
TAB-620LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000)
NC: Negative control PC: Positive control
Figure 3 Anti-Human IL23A Antibody (TAB-620LC) in ELISA
ELISA analysis of TAB-620LC was performed by coating with human IL23A protein. Then blocked with BSA and incubated with TAB-620LC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-human IL23A Recombinant antibody (TAB-620LC) in SDS-PAGE
SDS-PAGE analysis of TAB-620LC in non-reduced (Lane 1) and reduced (Lane 2) conditions.
Figure 5 Anti-human IL23A Recombinant antibody (TAB-620LC) in HPLC
HPLC analysis of TAB-620LC shows the purity is>95%.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-1953-VHH | Recombinant Anti-human IL23A VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
TAB-005LC-VHH | Anti-human IL23A Recombinant Antibody (TAB-005LC-VHH) | ELISA | Single domain antibody |
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-620LC, RRID: AB_3111979)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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