Chicken Anti-AKT1 (ab1) Polyclonal IgY

Name: Chicken Anti-AKT1 (ab1) Polyclonal IgY
SKU: BRD-0031MZ
Rating: 5 (1 reviews)

CAT#: BRD-0031MZ

This antibody is a chicken polyclonal antibody which specifically reacts with AKT1 (ab1).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human gall bladder

Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human cerebellum

Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg

Normal Tissue Figure 6 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg

Normal Tissue Figure 10 Testis

Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Cell line enhanced (MCF7)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1

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Specifications

Immunogen
Recombinant serine/threonine protein kinase

Host Species
Chicken

Type
Chicken antibody

Species Reactivity
Human, Mouse, Rat

Applications
WB

Target

Alternative Names
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Gene ID
207

UniProt ID
P31749

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Related Signaling Pathways

BCR Signaling Pathway

ErbB Signaling Pathway

Insulin Signaling Pathway

mTOR Signaling Pathway

PI3K-Akt Signaling Pathway

Ras Signaling Pathway

Related Diseases

Breast Cancer

Colorectal Cancer

Endometrial Cancer

Gastric Cancer

Hepatocellular Carcinoma

Non-small Cell Lung Cancer

Prostate Cancer

Endocrine Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - BRD-0031MZ.pdf

MSDS

MSDS - BRD-0031MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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CAT	Product Name	Application	Type
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CAT	Product Name	Application
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