Rabbit Anti-NHP AKT1 Recombinant Antibody (VS-1024-XY15)

Name: Rabbit Anti-NHP AKT1 Recombinant Antibody (VS-1024-XY15)
SKU: VS-1024-XY15
Rating: 5 (1 reviews)

CAT#: VS-1024-XY15

This rabbit-derived antibody is engineered to recognize AKT1 with high specificity. It is effective across human, mouse, and non-human primate samples. The antibody is generated using a synthetic peptide of AKT1 as the immunogen, which enhances its precision. Ideal for Western blot (WB) and immunoprecipitation (IP) applications, this reagent is a powerful tool for in-depth analysis of AKT1 signaling pathways, offering reliable performance in various experimental settings. Its versatility and accuracy make it an invaluable asset for advanced research and investigative studies.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human gall bladder

Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human cerebellum

Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg

Normal Tissue Figure 6 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg

Normal Tissue Figure 10 Testis

Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Cell line enhanced (MCF7)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1

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Specifications

Immunogen
Synthetic peptide

Host Species
Rabbit

Type
Rabbit IgG

Specificity
AKT1

Species Reactivity
Human, Mouse, Non-human primate

Applications
WB, IP

Product Property

Purification
Affinity chromatography

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
WB: 1:800, IP: 1:50

Target

Alternative Names
AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

Gene ID
207

UniProt ID
P31749

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Related Signaling Pathways

BCR Signaling Pathway

ErbB Signaling Pathway

Insulin Signaling Pathway

mTOR Signaling Pathway

PI3K-Akt Signaling Pathway

Ras Signaling Pathway

Related Diseases

Breast Cancer

Colorectal Cancer

Endometrial Cancer

Gastric Cancer

Hepatocellular Carcinoma

Non-small Cell Lung Cancer

Prostate Cancer

Endocrine Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY15.pdf

MSDS

MSDS - VS-1024-XY15.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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