What are the primary research applications for Anti-Human NT5E Recombinant Antibody?

The antibody is mainly used in research focused on tumor microenvironment studies and immunotherapy strategies. It is suitable for applications like flow cytometry, immunohistochemistry, and ELISA, where it aids in understanding NT5E's role in cancer cell biology.

Can this antibody help in studying diseases other than cancer?

Indeed, besides cancer, it's valuable in investigating autoimmune diseases and developmental biology, given NT5E's role in purine metabolism and cellular differentiation processes.

What makes Oleclumab suitable for complex assays?

Oleclumab's Fab fragment format allows reduced steric hindrance, facilitating better antigen access in complex surface protein studies. This improves binding efficiency in assays like surface plasmon resonance.

Does this antibody work with multiple species?

Although primarily designed for human NT5E, its cross-reactivity with similar isoforms in model organisms is currently under investigation, potentially extending its utility in comparative studies.

What are the known applications in inflammation research?

In inflammation research, this antibody can be used to map NT5E expression in inflamed tissues, shedding light on how ecto-5'-nucleotidase modulates immune response and inflammation resolution.

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NT5E
Anti-Human NT5E Recombinant Antibody (TAB-441CQ)

Anti-Human NT5E Recombinant Antibody (TAB-441CQ) (CAT#: TAB-441CQ)

Recombinant monoclonal antibody to NT5E. This product is a human monoclonal antibody that targets NT5E.

Gene Expression
Datasheet
MSDS
COA

Subcellular Location

Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg

Normal Tissue

Figure 2 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg

Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg

Figure 7 Cervix
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg

Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG1, λ

Specificity
Human 5'-nucleotidase

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies

Conjugate
Unconjugated

Related Disease
Solid Tumours, Pancreatic and Colorectal cancer

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Target

Alternative Names
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

Gene ID
4907

UniProt ID
P21589

Related Resources

Biosimilar Overview

Please refer to Oleclumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Oleclumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "NT5E"

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Human Antibody Products Blocking Antibody Products Rabbit Monoclonal Antibody Products Fab Antibody Products ADCC Enhanced Antibody Products ScFv Antibody Products IgG Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
TAB-593MZ	Human Anti-NT5E Recombinant Antibody (TAB-593MZ)	FC	Human IgG
TAB-594MZ	Human Anti-NT5E Recombinant Antibody (TAB-594MZ)	FC	Human IgG
TAB-595MZ	Human Anti-NT5E Recombinant Antibody (TAB-595MZ)	FC, FuncS	Human IgG
TAB-596MZ	Human Anti-NT5E Recombinant Antibody (TAB-596MZ)	FC	Human IgG
TAB-597MZ	Human Anti-NT5E Recombinant Antibody (TAB-597MZ)	FC	Human IgG

CAT	Product Name	Application	Type
NEUT-1799CQ	Recombinant Mouse Anti-NT5E Antibody (NEUT-1799CQ)	FC, IHC-Fr, IF, IP, Inhib	IgG2a, κ
NEUT-1800CQ	Recombinant Rat Anti-Nt5e Antibody (TY/23)	BL	IgG2a

CAT	Product Name	Application	Type
MOR-2503	Hi-Affi™ Recombinant Rabbit Anti-NT5E Monoclonal Antibody (DS2503AB)	WB, IHC, ICC	IgG

CAT	Product Name	Application	Type
HPAB-N0374-YC-F(E)	Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0374-YC-F(E))	ELISA, FC, Inhib	Mouse Fab
HPAB-N0375-YC-F(E)	Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0375-YC-F(E))	ELISA, FC, Inhib	Human Fab
HPAB-N0196-YC-F(E)	Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0196-YC-F(E))	FC	Human Fab
HPAB-0500-FY-F(E)	Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0500-FY-F(E))	Neut, FC	Mouse Fab
HPAB-0501-FY-F(E)	Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0501-FY-F(E))	Neut, FC	Mouse Fab

CAT	Product Name	Application	Type
AFC-TAB-441CQ	Afuco™ Anti-NT5E ADCC Recombinant Antibody, ADCC Enhanced	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-M0172-YC-S(P)	Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0172-YC-S(P))	FC, Inhib	Mouse scFv
HPAB-M0173-YC-S(P)	Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0173-YC-S(P))	FC, FuncS	Mouse scFv
HPAB-0500-FY-S(P)	Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0500-FY-S(P))	Neut, FC	Mouse scFv
HPAB-0501-FY-S(P)	Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0501-FY-S(P))	Neut, FC	Mouse scFv
HPAB-0502-FY-S(P)	Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0502-FY-S(P))	Neut, FC	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0500-FY	Mouse Anti-NT5E Recombinant Antibody (HPAB-0500-FY)	Neut, FC, Block, FuncS, WB	Mouse IgG
HPAB-0501-FY	Mouse Anti-NT5E Recombinant Antibody (HPAB-0501-FY)	Neut, FC, Block, FuncS, WB	Mouse IgG
PABJ-0226	Human Anti-NT5E Recombinant Antibody (PABJ-0226)	ELISA, Block	Human IgG
VS3-QX838	Mouse Anti-NT5E Recombinant Antibody (clone 974CT7.5.3)	WB, IHC	Mouse IgG1
VS3-XY1188	Mouse Anti-NT5E Recombinant Antibody (clone 4G6B10)	IHC	Mouse IgG1

CAT	Product Name	Application	Type
VS-0724-YC1442	AbPlus™ Anti-Nt5e Magnetic Beads (VS-0724-YC1442)	IP, Protein Purification
VS-0724-YC1493	AbPlus™ Anti-NT5E Magnetic Beads (VS-0724-YC1493)	IP, Protein Purification

Customer Reviews and Q&As

Customer Review Q&As

Consistent Performance Across Assays

We've used the Anti-Human NT5E Antibody across several platforms. It consistently performs well, providing reliable results. Its recombinant nature reduces variability, making it indispensable in our ongoing cancer research.

Superior for Tumor Studies

This antibody has been critical in our tumor profiling endeavors. By delivering precise targeting for NT5E, it has allowed us to derive insightful relationships between enzyme activity and tumor spread.

Enhancing Understanding of NT5E Interactions

Through co-immunoprecipitation techniques aided by this antibody, we've uncovered novel NT5E interaction partners, driving forward our understanding of its role in cellular signaling.

Flexible Use Across Protocols

Its flexible application across different assay types allows seamless integration into multiple research projects, from basic science to applied clinical research contexts.

Supports Robust Flow Cytometry Analysis

In flow cytometry, this antibody provides clear, specific signals, essential for discerning NT5E expression levels across cell populations with high accuracy.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-441CQ, RRID: AB_3111947)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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