Z-domain of protein A scaffold protein anti-Human CAIX
CAT#: SZA-L213
This CAIX specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=30 nM) for CAIX as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Normal Tissue
Figure 1 Immunofluorescent staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5100/639_D5_2_selected.jpg
Normal Tissue
Figure 2 Stomach
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55207/139851_A_5_1.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (A-431, HDLM-2, hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000107159-CA9
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Specifications
- Scaffold Name
- Z-domain of protein A
- Origin
- Staphylococcal protein A
- Core Structure
- 3-α helixes
- Variable Regions
- 13 Residues on first and second helix surface
- Target
- CAIX
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 30 nM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
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