Rabbit Anti-BDNF Recombinant Antibody (VS3-CJ801)
CAT#: VS3-CJ801
This product is a rabbit antibody that recognizes human, mouse, rat, and zebrafish BDNF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31440/1056_B9_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9564/162501_B_7_5.jpg
Normal Tissue
Figure 3 Cerebellum
GLUC cells - cytoplasm/membrane
Staining: Medium
Intensity: Moderate
Granular cells - cytoplasm/membrane
Staining: Medium
Intensity: Moderate
Molecular layer cells - cytoplasm/membrane
Staining: Medium
Intensity: Moderate
Molecular layer cells - nucleus
Staining: Medium
Intensity: Moderate
Processes in molecular layer
Staining: Medium
Intensity: Moderate
Synaptic glomeruli - core
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9564/162501_B_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HBF TERT88, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176697-BDNF
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Zebrafish BDNF
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- ANON2; BULN2
- Gene ID
- 627
- UniProt ID
- P23560
- Sequence Similarities
- Belongs to the NGF-beta family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- BDNF precursor form:
N-glycosylated and glycosulfated, contrary to mature BDNF.
Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF (PubMed:11152678).
Can be converted into mature BDNF by plasmin (PLG) (PubMed:19467646).
- Protein Refseq
- NP_001137277.1; NP_001137278.1; NP_001137279.1
- Function
- Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678).
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
BDNF precursor form:
Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994).
Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
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Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BDNF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-107 | Recombinant Anti-human BDNF Antibody | ELISA, WB, Dot, FuncS | IgG |
| MHH-107 | Recombinant Human Anti-human BDNF Antibody | ELISA, WB, FuncS | IgG |
| MRO-0166-CN | Rabbit Anti-BDNF Recombinant Antibody (clone CBACN-065) | WB, IHC, IF | Rabbit IgG |
| HPAB-0351-FY | Human Anti-BDNF Recombinant Antibody (clone R3BH1) | ELISA, Inhib, FuncS, Neut | Chimeric (chicken/human) IgG1, λ |
| VS3-CJ800 | Mouse Anti-BDNF Recombinant Antibody (VS3-CJ800) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-107-F(E) | Recombinant Anti-human BDNF Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-107-F(E) | Recombinant Human Anti-human BDNF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| HPAB-0351-FY-F(E) | Human Anti-BDNF Recombinant Antibody (clone R3BH1); Fab Fragment | Block | Chimeric (chicken/human) Fab, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-107-S(P) | Recombinant Anti-human BDNF Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| MHH-107-S(P) | Recombinant Human Anti-human BDNF Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| HPAB-0351-FY-S(P) | Chicken Anti-BDNF Recombinant Antibody (clone R3BH1); scFv Fragment | Block | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1247CL | Anti-Human BDNF Recombinant Antibody (B30) | Inhib, FuncS | Humanized antibody |
| TAB-1248CL | Anti-Human BDNF Recombinant Antibody (B20) | Inhib, FuncS | Humanized antibody |
| TAB-1247CL-S(P) | Anti-Human BDNF Recombinant Antibody scFv Fragment (B30) | Inhib, FuncS | Humanized antibody |
| TAB-1248CL-S(P) | Anti-Human BDNF Recombinant Antibody scFv Fragment (B20) | ELISA, Inhib | Humanized antibody |
| TAB-1249CL-S(P) | Anti-Human BDNF Recombinant Antibody scFv Fragment (B18) | ELISA, Inhib | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0825MZ | Chicken Anti-BDNF Polyclonal IgY | ELISA | Chicken antibody |
| BRD-1149MZ | Chicken Anti-Human BDNF Polyclonal IgY | ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-044CQ | Mouse Anti-BDNF Recombinant Antibody (clone CBL263) | ELISA, WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0351 | Hi-Affi™ Rabbit Anti-BDNF Recombinant Antibody (clone DS351AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC576 | Recombinant Anti-BDNF Vesicular Antibody, EV Displayed (VS-0425-YC576) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY753 | Anti-BDNF Immunohistochemistry Kit | IHC | |
| VS-0525-XY754 | Anti-Mouse BDNF Immunohistochemistry Kit | IHC |
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