Rabbit Anti-CD27 Recombinant Antibody (VS-0723-WK285) (CAT#: VS-0723-WK285)
This product is a rabbit IgG antibody that recognizes human CD27, which plays a key role in regulating B-cell activation and immunoglobulin synthesis. This antibody is recommended for flow cytometry (1/100) and ELISA (1/5000-1/10000) assays.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Figure 2 Tonsil
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
Figure 3 High expression in lymph node.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_7_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_7_8_val_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Figure 5 Tonsil
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
Figure 6 RNA cell line category: Group enriched (Daudi, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139193-CD27
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139193-CD27
Figure 3 RNA cell line category: Group enriched (Daudi, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139193-CD27
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139193-CD27
Specifications
- Immunogen
- Recombinant human CD27 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD27
- Species Reactivity
- Human
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 1×PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/5000-1/10000
Flow Cytometry: 1/100
Target
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Human Antibody
Mouse Antibody
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Humanized Antibody
Neutralizing Antibody
Rabbit Monoclonal Antibody
Agonistic Antibody
Recombinant Antibody
scFv Fragment Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H74 | Anti-Human CD27 Recombinant Antibody (Varlilumab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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