Mouse Anti-CD7 Recombinant Antibody (ZG-0133J)
CAT#: ZG-0133J
This product is a mouse antibody that recognizes CD7.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CD7 Recombinant Antibody (ZG-0133J) in IHC
Immunohistochemical analysis of paraffin-embedded Tonsil . 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-CD7 Recombinant Antibody (ZG-0133J) in IHC
Immunohistochemical analysis of paraffin-embedded Tonsil. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
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Normal Tissue
Figure 1 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39079/133943_A_8_3.jpg
Normal Tissue
Figure 2 Spleen
Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39079/133943_B_7_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Hep G2, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173762-CD7
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Specifications
- Immunogen
- Synthesized peptide derived from human CD7
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD7
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
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Clear Staining On Paraffin Sections
We utilized this recombinant mouse antibody for Immunohistochemistry on paraffin-embedded sections (IHC-P) to detect CD7 in thymic tissue. The antibody yielded clear membrane staining on T-cells as expected. The signal was robust, allowing us to easily distinguish lymphoid populations in the tissue architecture.
Effective For Immunofluorescence
This antibody also worked well in our Immunofluorescence assays on paraffin sections. It specifically recognized human CD7, helping us study T-cell/B-cell interactions. The fact that it is a recombinant mouse IgG adds a layer of consistency to our imaging experiments that we greatly value.
Q&As
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Can this antibody be used on paraffin-embedded tissues?
A: Yes, this product is validated for Immunohistochemistry-Paraffin (IHC-P) and Immunofluorescence on paraffin sections (IF). It is a mouse antibody that recognizes Human CD7, a transmembrane protein found on thymocytes and mature T cells.
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What are the storage recommendations for this antibody?
A: For short-term use, the antibody can be stored at 4°C. For long-term storage, we recommend storing it at -20°C to ensure stability. Please avoid repeated freeze/thaw cycles, as this can be detrimental to the antibody's performance.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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