Mouse Anti-CD7 Recombinant Antibody (clone RFT2) (CAT#: FAMAB-0050CQ)

This product is a recombinant mouse antibody clone RFT2, which specifically binds to CD7.

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Inhib

Figure 1 Increasing amounts (0.05-2 μg/ml) of RFT2 causes increasing inhibition of the T cell response to tuberculin PPD.

Figure 1 Increasing amounts (0.05-2 μg/ml) of RFT2 causes increasing inhibition of the T cell response to tuberculin PPD.

The response is shown as a percentage of that in the presence of PPD alone and represents the results of four experiments. NS. not significant; *<0.03; +P< 0.06; ++<0.009.

Costantinides, Y., Kingsley, G., Pitzalis, C., & Panayi, G. S. (1991). Inhibition of lymphocyte proliferation by a monoclonal antibody (RFT2) against CD7. Clinical & Experimental Immunology, 85(1), 164-167.

FuncS

Figure 2 The effect of RFT2 (0; 0.05; 0.2 and 2.0 μg/ml) Oh the proliferation of T cells from control subjects induced by phylohucmagglulinin (PHA), concanavalin A (ConA) phorbol myristate actate (PMA) and OKT3.

Figure 2 The effect of RFT2 (0; 0.05; 0.2 and 2.0 μg/ml) Oh the proliferation of T cells from control subjects induced by phylohucmagglulinin (PHA), concanavalin A (ConA) phorbol myristate actate (PMA) and OKT3.

The results from four separate experiments are shown and the bars represent the means. *P

Costantinides, Y., Kingsley, G., Pitzalis, C., & Panayi, G. S. (1991). Inhibition of lymphocyte proliferation by a monoclonal antibody (RFT2) against CD7. Clinical & Experimental Immunology, 85(1), 164-167.

FuncS

Figure 3 Mononuclear cells (MNC) incubated with RFT2 (a) or an irrelevant monoclonal antibody (b) were washed and added at various number to fresh MNC which were stimulated with PPD.

Figure 3 Mononuclear cells (MNC) incubated with RFT2 (a) or an irrelevant monoclonal antibody (b) were washed and added at various number to fresh MNC which were stimulated with PPD.

The results of four experiments are shown. *P<0.03 when compared with cultures containing the same number of MNC both fresh and pre-treated with the irrelevant monoclonal antibody. All other differences are not significant.

Costantinides, Y., Kingsley, G., Pitzalis, C., & Panayi, G. S. (1991). Inhibition of lymphocyte proliferation by a monoclonal antibody (RFT2) against CD7. Clinical & Experimental Immunology, 85(1), 164-167.

FuncS

Figure 4 The expression of interlcukin-2 receptor (IL-2R) on the surface of T cells stimulated with tuberculin PPD for 2 days or 5 days in the presence or absence of RFT2. The results of three separate experimenis are shown.

Figure 4 The expression of interlcukin-2 receptor (IL-2R) on the surface of T cells stimulated with tuberculin PPD for 2 days or 5 days in the presence or absence of RFT2. The results of three separate experimenis are shown.

Costantinides, Y., Kingsley, G., Pitzalis, C., & Panayi, G. S. (1991). Inhibition of lymphocyte proliferation by a monoclonal antibody (RFT2) against CD7. Clinical & Experimental Immunology, 85(1), 164-167.

IHC

Figure 5 Immunohistologic specificity of the binding of RFT2 (A and B) and CHT2 (C and D) on normal thymus(A and C) and tonsil (B and D).

Figure 5 Immunohistologic specificity of the binding of RFT2 (A and B) and CHT2 (C and D) on normal thymus(A and C) and tonsil (B and D).

Note the identical reactivity pattern and the simiby both mAb. Arrows point to very larly wide range of staining intensity seen strongly positive T blast cells inslde the thymic cortex. In Band D the broken line delineates the boundary between the follicle with germinal center (gc) and the T cell-rich paracortical region (PC). ascertained by double staining for IgM deposits in the germinal center (not shown). Apart from T lineage cells no other cell types In the body are labeled with CHTP.

Heinrich, G., Gram, H., Kocher, H. P., Schreier, M. H., Ryffel, B., Akbar, A., ... & Janossy, G. (1989). Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. The Journal of Immunology, 143(11), 3589-3597.

IF

Figure 6 Triple immunofluorescent analysis of RFTP (E and D) and CHT2, reactivity(C and E) of blood lymphocytes expressing CD45 R (LCA, p220.200: A, B, and C) and UCHLl (LCA. p180: A. D, and E).

Figure 6 Triple immunofluorescent analysis of RFTP (E and D) and CHT2, reactivity(C and E) of blood lymphocytes expressing CD45 R (LCA, p220.200: A, B, and C) and UCHLl (LCA. p180: A. D, and E).

The lymphocyte populations were gated on the forward angle and side scatterby using a FACScan flow cytometer. The three immunofluroescent channels included DuoCHROME (DC; UCHL1-biotin with streptavidin second layer) phycoerythrin (PE; directly labeled CD45R) and FITC. The latter was either used directly (RFT2-FITC) or indirectly (CHT2 with goat antihuman-Ig-FITC). The B lymphocyte populations (strongly CD45R+. but UCHLI- RFT2-) shown in boxes represent 7.0% of lymphocytes counted.

Heinrich, G., Gram, H., Kocher, H. P., Schreier, M. H., Ryffel, B., Akbar, A., ... & Janossy, G. (1989). Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. The Journal of Immunology, 143(11), 3589-3597.

FC

Figure 7 CD7 expression detected by RFTP (a) and CHTP (b) on T lymphoid populations.

Figure 7 CD7 expression detected by RFTP (a) and CHTP (b) on T lymphoid populations.

(A) unstimulated UCHLl+ T lymphocytes; (B) unstimulated CD45R+ T cells; (C) T-ALL blast cells; (D) lymphoblasts from 64-h PHA stimulated cultures. Note that "A" include CD7- and CD7± cells, "B" include CD7+ cells, whereas both T-ALL blasts and T lymphoblasts show even stronger positivity. RFTP was conjugated to FITC and CHTPbiotin was detected with streptavidin-FITC

Heinrich, G., Gram, H., Kocher, H. P., Schreier, M. H., Ryffel, B., Akbar, A., ... & Janossy, G. (1989). Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. The Journal of Immunology, 143(11), 3589-3597.

Cyt

Figure 8 ADCC using CF⁵¹-labeled T-ALL (a and b, two cases) or PHA blasts (c).

Figure 8 ADCC using CF⁵¹-labeled T-ALL (a and b, two cases) or PHA blasts (c).

Effector cells were PBMC from healthy donors. Target cells were coated with CHTP, RFTZ or uncoated by using a control irrelevant mAb at 1 μg/ml 10⁸ cells /ml.

Heinrich, G., Gram, H., Kocher, H. P., Schreier, M. H., Ryffel, B., Akbar, A., ... & Janossy, G. (1989). Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. The Journal of Immunology, 143(11), 3589-3597.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human CD7
  • Species Reactivity
  • Human
  • Clone
  • RFT2
  • Applications
  • IHC, FC, Block, IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Immunohistochemistry, Flow Cytometry, Blocking, Immunofluorescence, Cytotoxicity and Functional Assay.

Target

  • Alternative Names
  • CD7 Molecule; T-Cell Leukemia Antigen 2; T-Cell Surface Antigen Leu-9; CD7 Antigen (P41); P41 Protein; GP40; TP41; T-Cell Antigen CD7; CD7 Antigen; LEU-9; Tp40

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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